UniOne® TR-FRET Human IL10/IL10RA Binding Kit
SKU: 91944727814

UniOne® TR-FRET Human IL10/IL10RA Binding Kit

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Description

UniOne® TR-FRET Human IL10/IL10RA Binding KitProduct Specification Host Human Stability & Storage 80 Background This kit utilizes HomogeneousTime Resolved Fluorescence (TR FRET) technology to measure the interactionbetween IL10A and IL10RA. This method enables simple and rapid high throughputscreening of inhibitors and antibody blockers. As illustrated, the interactionbetween IL10A and IL10RA is detected using an Eu labeled Anti Tag1 antibody(TR FRET donor) and an Acceptor labeled IL10A protein

Product Specification


Host Human
Stability & Storage

-80℃

Background

This kit utilizes HomogeneousTime-Resolved Fluorescence (TR-FRET) technology to measure the interactionbetween IL10A and IL10RA. This method enables simple and rapid high-throughputscreening of inhibitors and antibody blockers.

As illustrated, the interactionbetween IL10A and IL10RA is detected using an Eu-labeled Anti-Tag1 antibody(TR-FRET donor) and an Acceptor-labeled IL10A protein (TR-FRET acceptor). Thebinding of IL10A to IL10RA brings the donor antibody and the acceptor proteininto close proximity. Excitation of the donor antibody then triggersFluorescence Resonance Energy Transfer (FRET) to the acceptor antibody,resulting in the emission of a specific signal at 665nm. The intensity of thisspecific signal is proportional to the extent of the IL10A and IL10RAinteraction. This homogeneous assay is simple to perform and requires nowashing steps.



Components

Kit Components】

Component

Concentration

500 TESTS

2500 TESTS

10000 TESTS

Storage Temp.

IL10A-01A protein

100×

25μL

125μL

500μL

-80°C

Tag1- IL10 RA protein

100×

40μL

200μL

800μL

-80°C

Anti-Tag1 Eu dye antibody

50×

50μL

250μL

1000μL

-80°C

Detection buffer

10×

2mL

10mL

40mL

-80°C

*Reagents should be protected from repeated freeze-thaw cycles. It is recommended to aliquot upon first thaw and store according to the specified conditions. The shelf life is one year.

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SKU: 91944727814

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VALENCA NOVOA
West Palm Beach, US
★★★★★ 5
I Loved It
Format: Kindle
This was my first time reading a book by Elle Alexanda, and it definitely won’t be my last! I was absolutely captivated by the Lou/Ava love story. I found Lou’s vulnerability so endearing. It was an excellent read, and I wish it were available as an audiobook. The story was a slow burn, but it kept me hooked. The flirting and banter between Lou and Ava were so entertaining. I didn’t want the story to end! Well done, Elle!
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Reviewed in the United States on May 21, 2025
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Hailey Watkins
Cuba, US
★★★★★ 4
Romance with a side of Thrill
Format: Kindle
4.25 stars! I was immediately engrossed in this debut novel, with the thrilling beginning and obvious future romance between Lou and Ava. I thought the setting of the town was wonderful, full of small charm and supportive characters. The little nods towards the continuing threat to Ava’s life were good. The budding romance was great, too! I think the intimacy scenes were soft and perfect. The main reason this didn’t reach 4.5 stars for me (thus would’ve rounded it to 5 instead of down to 4), is because of the POV switches during chapters. They’re a bit jumbled in; the reader is suddenly seeing or hearing things from Lou when it started with Ava, but then it quickly switches back to Ava. It’s not my favorite way for a story to be shared. I also lost a little steam towards the middle-end, right before the big finale. I’m not sure if my other hobby distracted me or if the story hit a slow spot. It’s possible I was hoping for a little more excitement. This story had more romance than thrill.
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Reviewed in the United States on July 21, 2025
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MLTU
San Leandro, US
★★★★★ 5
Blood and Muscle
Format: Kindle
I had this on my shelf for a while. lG reviews were great, the cover was pretty, and it sounded really interesting. It was better than I expected! Love when that happens🥰 The story was great, the writing was good, and the characters were amazing! Ava and Lou's chemistry at first was a little charged. But as the story went and their relationship developed, it turned into something more tender and very intimate. Throw in the witness protection thing, and you have quite exciting romance. I will be reading this again!
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Reviewed in the United States on September 7, 2025
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C. David
Houston, US
★★★★★ 5
Cute and easy read
Format: Kindle
The story is cute and easy read.....just a slight bit of drama...nothing to heart wrenching. Characters are cute and very likeable...some spice.... Good ready if you just need a quick and easy story with mild spice
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Reviewed in the United States on March 5, 2025
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R S Jordan
Cuba, US
★★★★★ 4
Thrilling Fusion of Suspense and Sapphic Longing: Blood and Muscle Delivers with Depth and Desire
Format: Kindle
Elle Alexandra delivers a masterfully woven tale of suspense and romance in Blood and Muscle, a novel that seamlessly balances pulse-pounding tension with deeply felt emotional intimacy. Set against the stark yet breathtaking backdrop of Cedar Falls, Wyoming, this story immerses readers in a world where danger and desire are inextricably linked, and where trust is both a luxury and a necessity. At the heart of the novel are Ava Bedford, a reluctant fugitive thrust into witness protection after witnessing a murder tied to a powerful political figure, and Sheriff Lou Harman, a woman whose life is defined by structure, solitude, and an unwavering sense of duty. Their dynamic is as compelling as it is electrifying, Ava’s sharp wit and warmth slowly chipping away at Lou’s carefully maintained defenses, creating a relationship that simmers with slow-burning intensity. Alexandra’s character work is nothing short of exquisite. Both Ava and Lou are richly developed, imbued with layers of vulnerability, resilience, and undeniable magnetism. Their interactions feel organic, their chemistry electric, and their individual arcs deeply satisfying. The town of Cedar Falls, too, becomes more than just a setting, it breathes with its own quiet menace and rugged charm, a place of both refuge and lurking danger. The novel’s pacing is expertly handled, with the tension mounting in a way that keeps readers perpetually on edge. Alexandra deftly balances moments of tender introspection with sequences of gripping suspense, ensuring that neither the romance nor the thriller elements overshadow the other. The prose is fluid, evocative, and often poetic, drawing readers deeper into the world she has so carefully crafted. If there is one minor drawback, it is the at-times subtle transition between the protagonists’ perspectives. While both Ava and Lou’s voices are distinct, a more clearly delineated shift in POV would have further enhanced the reading experience, offering even greater immersion into their respective inner worlds. However, this is a small quibble in an otherwise exceptional novel. Blood and Muscle is a triumph, an intoxicating blend of romance, intrigue, and raw emotion. It is a testament to the power of resilience, the fragility of trust, and the courage it takes to love in the face of uncertainty. Elle Alexandra has crafted a story that lingers long after the final page, making this a must-read for fans of sapphic fiction who crave both heart-pounding suspense and soul-stirring romance.
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Reviewed in the United States on March 11, 2025

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