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Description
Mouse CXCL10/IP-10/CRG-2 ELISA KitProduct Specification Theory Double antibody sandwich enzyme linked immunosorbent assay was used in this kit. Specific anti mouse CXCL10 IP 10 CRG 2 antibodies were precoated on high affinity microplate. After incubation, CXCL10 IP 10 CRG 2 present in the sample combined with the solid phase antibody and the detection antibody to form an immune complex. After washing to remove the unbound material, the horseradish peroxidase labeled Streptavidin HRP
Product Specification
| Theory | Double antibody sandwich enzyme-linked immunosorbent assay was used in this kit. Specific anti-mouse CXCL10/IP-10/CRG-2 antibodies were precoated on high-affinity microplate. After incubation, CXCL10/IP-10/CRG-2 present in the sample combined with the solid-phase antibody and the detection antibody to form an immune complex. After washing to remove the unbound material, the horseradish peroxidase labeled Streptavidin-HRP was added. After washing, the chromogenic substrate was added and the color was developed in the dark. Join terminated liquid termination reaction, in the 450 - nm wavelength (reference calibration wavelength of 540 nm or 570 nm) determination of absorbance values. | |||||||||||||||||||||||||||
| Composition |
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| Background | Chemokine (C - X - C motif) ligand 10 (CXCL10) belongs to CXC chemokines family a small cytokines, also known as 10 - kda interferon gamma induced proteins (beta IP10 or IP - 10). In response to IFN-α, CXCL10 is secreted by several cell types. These cell types include monocytes, endothelial cells, and fibroblasts. CXCL10 is thought to have a variety of effects, such as chemoattractant to monocytes and T cells, promotion of T cell adhesion to endothelial cells, anti-tumor activity and inhibitory effects on bone marrow colony formation and angiogenesis. IP-10 was originally identified as an IFN-γ-inducible gene in monocytes, fibroblasts and endothelial cells. CRG-2, the mouse homolog of human IP-10, shares approximately 67% amino acid sequence identity with human IP-10. | |||||||||||||||||||||||||||
| General Notes | 1. Please use the kit within the validity period. 2. Components of different kits and different batch kits should not be mixed. 3. If the sample value is greater than the highest value of the standard curve, the sample should be diluted with (1×) The samples were diluted and retested. "If the cell culture supernatant sample needs to be diluted in a distributed manner, cell culture medium may be used for intermediate dilutions, except for the last step when diluent is used." 4. The difference of the test results can be caused by a variety of factors, including the operation of the experimenter, the use of the pipettor, the washing technique, the reaction time or temperature, the storage of the kit, etc. 5. The termination solution in the kit is acidic. Please protect your glasses, hands, face and clothes when using it. 6. For scientific research only, not for in vitro diagnosis. |
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| Storage Temp. | Unopened kit, 2-8° C Storage | |||||||||||||||||||||||||||
| Test Range | 62.5pg/mL-4000pg/mL |
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4.0 ★★★★★
Based on 22 reviews
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Product Reviews
★★★★★ 5
My dog LOVES this
Style: Goal Post Chew Toy, Color: Dallas Cowboys
I originally purchased 1 of these at Walmart. My dog did his thing with it and loved it so much that I had to repurchase another one through Amazon. My dog LOVES THIS TOY! It's his favorite!
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Reviewed in the United States on January 7, 2026
★★★★★ 5
Great toy
Style: Goal Post Chew Toy, Color: Dallas Cowboys
My dog loves it and has is quickly becoming her fav toy. I love how sturdy it for my power chewer.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on January 8, 2026
★★★★★ 5
It's Pittsburgh Steelers!!
Style: Goal Post Chew Toy, Color: PITTSBURGH STEELERS
I ordered it for our "Grandpups"! My daughter says it's tuff & strong but at the same time it gives enough for the dogs to bite down on it. They love it!! I ordered it along with a few Steeler bandanas!
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Reviewed in the United States on March 15, 2026
★★★★★ 3
Not for the serious chewers
Style: Goal Post Chew Toy, Color: NEW ENGLAND PATRIOTS
Great novelty dog toy but if your dog is a real chewer like my Frenchie is, he had this turned this into a weapon in a matter of a week. The chewed up plastic was super sharp! But other than that, Go Pats!
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Reviewed in the United States on October 9, 2025
★★★★★ 5
Buy it!!
Style: Goal Post Chew Toy, Color: Dallas Cowboys
It quickly became my guy's dog's favorite toy. Sturdy, cute, and affordable. I ordered another one
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Reviewed in the United States on January 5, 2026