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Description
Human Breast Cancer Organoid Culture Medium KitProduct Specification Usage 1. Primary (1) The extracted tissue must be placed in a pre cooled (2 8 C) tissue preservation solution E sampling bottle, quickly transported to a clean laboratory for tissue treatment and cell separation, photographed, and registered with information (2) Prepare several culture dishes and add 4 pre cooled primary culture buffer B for later use (3) Disinfect the sampling bottle, place the tissue in a culture dish, clean it
Product Specification
| Usage |
1. Primary (1) The extracted tissue must be placed in a pre cooled (2-8° C) tissue preservation solution E sampling bottle, quickly transported to a clean laboratory for tissue treatment and cell separation, photographed, and registered with information (2) Prepare several culture dishes and add 4 ℃ pre cooled primary culture buffer B for later use (3) Disinfect the sampling bottle, place the tissue in a culture dish, clean it three times with primary culture buffer B, and then use an ophthalmic scissors or surgical knife to cut the tissue into tissue blocks with a volume of approximately 1-3mm (4) The tissue was digested with the digestive fluid C of pig normal lung primary tissue, and shaken at 37 ℃ for 20-25 minutes (observe the digestion process at any time) (5) Take a small amount of liquid and observe under a microscope. After observing a large number of individual cells or cell clusters below 70um, add three times the volume of primary culture buffer B to terminate digestion (6) Filter using a 100um pore sieve, collect the filtrate, concentrate and centrifuge at 300g for 5 minutes, remove the supernatant, add primary culture buffer B, and resuspend and centrifuge (7) Matrix adhesive calculation: After step 6, observe the collected tissue volume and add 25 times the tissue volume of matrix adhesive (ABS9495) to resuspend the board 2. Organ like passage culture (1) Pipette the culture medium and add 1-2ml of 4 ℃ organ like passage culture buffer G to each well for 2 minutes (2) Gently blow the matrix adhesive with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10 minutes. (Each 6-8 wells is a group) (3) a: If the number of organoids is insufficient or the volume is small, centrifuge for 5 minutes to discard the supernatant. Add an appropriate amount of organoid passage culture buffer G and resuspend it into a 1.5ml centrifuge tube. Centrifuge for 5 minutes to discard the liquid and proceed to step 4  & Nbsp& Nbsp& Nbsp& Nbsp; b: When the number of organoids is large or the volume is large: centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid passage digestion solution D for 2-3 minutes, add organoid passage culture buffer G to terminate digestion, centrifuge for 5 minutes to discard the mixture, add an appropriate amount of organoid passage culture buffer G and resuspend into a 1.5ml centrifuge tube, centrifuge for 5 minutes to discard the liquid for step 4 3. Organ like cryopreservation (1) Pipette the culture medium and add 1-2ml of 4 ℃ organ like passage culture buffer G to each well for 2 minutes (2) Gently blow the matrix adhesive with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10 minutes. (Each 6-8 wells is a group) (3) Centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid passage culture buffer G, and resuspend again. Centrifuge for 5 minutes at 300g to discard the liquid (4) Add an appropriate amount of organoid cryopreservation solution F, gently blow and resuspend, taking a 24 well cell culture plate as an example: the density is 2 wells, and one tube is frozen, with a volume of 1.4ml per tube (5) Mark the information properly, cool down the program, and then transfer it to liquid nitrogen for long-term storage 4. Organ like resuscitation (1) Take 10ml of organ like passage culture buffer G and place it in a 15ml centrifuge tube (2) Remove frozen organoid cells from a liquid nitrogen tank and quickly melt them in a 37 ℃ water bath(5) Suspension of matrix adhesive, with 25 ul of matrix adhesive per well laid on a 24 well cell culture plate, placed in a culture chamber for 10-15 minutes to form gel, and added with 500 ul of pig normal lung organ culture medium A. |
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| Description | Composition:
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| Storage Temp. | 4 ℃, 3months-20 ℃, 1 year, see the label for details |
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4.3 ★★★★★
Based on 28 reviews
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Product Reviews
★★★★★ 5
Perfect
Size: Large, Color: Calypso Blue
Love these! Perfect fit. Ordered normal size! They are a bit loose but look great that way! Very comfortable!They are perfect length at my ankles and I'm 5'3"
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Reviewed in the United States on May 3, 2026
★★★★★ 5
Worthy
Size: Small, Color: Calypso Blue
I have to say, I seldom receive something thru Amazon that's 100% worthy! I wear F P & AE & these look just like the model! I usually wear a M, but knowing how fp sizing is, I got the S. Fits perfect with even a bit of room. Im 5'3", 127lbs, 27 waist. Definitely great with flats, but I'm not sure yet with any other than tennis shoes. The paint splatter on mine are less than picture....no complaint here. They have a bit of stretch, I like real jeans, so I can live with it. They are soft & actually made well. Only downside to this order, Amazon made me purchased over what I wanted to, to get it by promise date, never happen & getting very common with that trick, not appreciated, so beware you may not get them on promise date
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Reviewed in the United States on April 4, 2026
★★★★★ 3
Way to short, cute, but short
Size: Medium, Color: Green Multi Combo
I would give these a 5 star but they were 3 inches to short. So cute though. Will try a large to see if that makes a difference. Im 5'7" 147lbs. Everything else was a great fit but serious highwaters
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Reviewed in the United States on June 1, 2026
★★★★★ 5
Super cute baggy jeans
Size: Small, Color: Calypso Blue, Size: Small, Color: Calypso Blue
I am short and these jeans are like normal jeans for me. 5’1” and size small ordered. I love these jeans!! They are baggy and have a good stretch. They have a cute drawstring as well as a belt. Plenty of pockets. Comfy and soft denim plus zippers are good too. Recommended!!
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Reviewed in the United States on March 28, 2026
★★★★★ 5
Great fit
Size: Medium, Color: Calypso Blue
Love these jeans, so loose and comfy and cute!
I’m 130 lbs 5’2, I ordered a medium.
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Reviewed in the United States on May 26, 2026