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Description
Human vitamin C ELISA KitProduct Specification Usage Specimen requirements: 1. Extract specimens as soon as possible after collection, and the extraction should be carried out according to relevant literature. Experiments should be carried out as soon as possible after extraction. If the test cannot be carried out immediately, the specimen can be stored at 20 , but repeated freezing and thawing should be avoided 2. Samples containing NaN3 cannot be detected because NaN3
Product Specification
| Usage | Specimen requirements: 1. Extract specimens as soon as possible after collection, and the extraction should be carried out according to relevant literature. Experiments should be carried out as soon as possible after extraction. If the test cannot be carried out immediately, the specimen can be stored at-20 ℃, but repeated freezing and thawing should be avoided 2. Samples containing NaN3 cannot be detected because NaN3 inhibits the activity of horseradish peroxidase (HRP). Operation steps: 1. Dilution of standard product: This kit provides one original standard product. Users can dilute it in a small test tube according to the following chart.
2. Sample addition: Set up blank wells (the blank control wells do not add samples and enzyme-labeled reagents, and the other steps are the same), standard wells, and sample wells to be tested respectively. Accurately add 50uL of the standard on the enzyme-labeled coated plate, first add 40uL of sample diluent to the sample well to be tested, and then add 10uL of the sample to be tested (the final dilution of the sample is 5 times). Add the sample to the bottom of the well of the enzyme labeled plate, try not to touch the well wall, and gently shake and mix well. 3. Incubation: Incubate at 37 °C for 30 minutes after sealing with a plate sealing film. 4. Liquid preparation: 30 times concentrated washing solution is diluted 30 times with distilled water and used for later use 5. Washing: Carefully remove the sealing film, discard the liquid, spin dry, fill each hole with washing liquid, let it stand for 30 seconds and then discard it. Repeat this 5 times and pat dry. 6. Enzyme addition: 50uL of enzyme-labeled reagent is added to each well, except for blank wells. 7. Incubation: The procedure is the same as 3. 8. Washing: The operation is the same as 5. 9. Color development: First add 50uL of color developer A to each well, then add 50uL of color developer B, gently shake and mix well, and develop color at 37 °C in the dark for 10 minutes. 10. Termination: Add 50uL of stop solution to each well to terminate the reaction (blue immediately turns yellow at this time). 11. Measurement: Zero the blank well, and measure the absorbance (OD value) of each well sequentially at a wavelength of 450nm. The determination should be performed within 15 minutes after the addition of the stop solution. Calculation: Taking the concentration of the standard substance as the abscissa and the OD value as the ordinate, draw a standard curve on the coordinate paper, and find out the corresponding concentration from the standard curve according to the OD value of the sample. Multiply by the dilution factor. Or use the concentration of the standard substance and the OD value to calculate the linear regression equation of the standard curve, substitute the OD value of the sample into the equation, calculate the sample concentration, and then multiply it by the dilution factor, which is the actual concentration of the sample. |
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| Species Reactivity | Human | ||||||||||||||||||||||||
| Theory | This kit uses double antibody sandwich method to determine the level of human vitamin C in specimens. Coating a microwell plate with purified human vitamin C antibody to make a solid phase antibody, adding vitamin C into the microwells coated with monoclonal antibody sequentially, and then combining with HRP-labeled vitamin C antibody to form an antibody-antigen-enzyme-labeled antibody complex, and adding substrate TMB to develop color after thoroughly washing. TMB is converted to blue under the catalysis of HRP enzyme and to the final yellow under the action of acid. There was a positive correlation between the depth of color and the vitamin C in the sample. The absorbance (OD value) was measured with a microplate reader at 450nm wavelength, and the concentration of human vitamin C in the sample was calculated by the standard curve. | ||||||||||||||||||||||||
| Synonym | Human VC ELISA Kit | ||||||||||||||||||||||||
| Composition | Kit composition:
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| General Notes | 1. The kit should be taken out of the refrigerated environment and balanced at room temperature for 1 hour before it can be used. If the enzyme-labeled coated plate is not used up after opening, the strips should be stored in a sealed bag. 2. Crystals may precipitate in the concentrated washing liquid. When diluting, it can be heated in a water bath to help dissolve, and the results will not be affected during washing. 3. A sampler should be used in each step of sampling, and its accuracy should be checked frequently to avoid test errors. It is best to control the sample addition time within 5 minutes. If the number of specimens is large, it is recommended to use a row gun to add samples. 4. Please make a standard curve at the same time of each measurement, preferably a double hole. If the content of the substance to be tested in the sample is too high (the OD value of the sample is greater than the OD value of the first well of the standard well), please dilute it with the sample diluent for a certain multiple (n times) before measuring, and please finally multiply it by the total dilution multiple (× n × 5) when calculating. 5. The sealing film is only for one-time use to avoid cross-contamination. 6. Please keep the substrate away from light. 7. Strictly follow the instructions, and the test results must be determined based on the reading of the microplate reader. 8. All samples, washing solutions and various wastes should be treated as infectious agents. 9. Components of different batch numbers of this reagent shall not be mixed. |
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| Storage Temp. | Store at 2-8 °C, shelf life 6 months. | ||||||||||||||||||||||||
| Test Range | 6ug/L -225ug/L | ||||||||||||||||||||||||
| Applications | It is used to determine the content of vitamin C in human serum, plasma and related fluid samples. |
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4.5 ★★★★★
Based on 10 reviews
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Product Reviews
★★★★★ 5
Very detailed! Beautiful world building! Strong Heroine!
Format: Kindle
Starts off a little slow and confusing with different POV’s, but starts to all come together towards the middle to make an elaborate plot line and makes it all worth it. Beautiful world building and attention to detail as well as great writing. The cliffhanger was gut wrenching! Can’t wait for the next book!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on March 4, 2020
★★★★★ 4
Interesting
Format: Kindle
This was a very captivating book once you got into it thoroughly. But the third person perspective was a bit hard to get used to. But as you got into it and followed the different characters, it was interesting and filled with intrigue, conflict and forbidden love.
I can’t wait to read the next one and to complete the series.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on November 12, 2022
★★★★★ 5
OUTSTANDING
Format: Kindle
This was very, very good. The world is vast and characters are complex. There is a good plot with a whole lot going on. This is well written. Good twists and turns and some heart breaking moments. You will love these characters, they have heart and loyalty. I am hoping that there will be several more books. We've yet to see anything from the Sea Court but only a mention of them here and there. The Wood Court was given a quick couple of scenes, and only as far as some warriors, we've yet to enter their court and the Shadow Court, I'm not sure if they will be a force for good or bad, but they definitely will play a much bigger role moving forward. This is primarily the Ice and Air Courts. Told in multiple views, which I loved, it gives you a chance to see things from different eyes. There's alot of political maneuvering and deception. I loved it and will pick up the next book as it becomes available. If you like The Fae and the courts, you should love this. I think the author has mucn in store for us.
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Reviewed in the United States on March 8, 2020
★★★★★ 4
Definitely worth the read!
Format: Kindle
After taking a deep breath and taking in that wicked twist of an ending, I have finally composed myself. My first thought when I started this book was that I love Reyna's character. I was intrigued by her connection with her familiar and the Ruin that is plaguing her land. It came as no surprise that she took her sister's place in an attempt to protect her. When they reach the Air Court everything slows down. This is where it was iffy for me. First of all, I like multiple POV's in books however 7 is a bit much. It starts to interrupt the story line. I felt like I was finally making progress connecting with one character, then it was switched to another person. I felt they all had necessary or pertinent information but not necessarily were they all POV worthy. The only other thing that annoyed me was that Reyna constantly was " trapped." She would rush off without thinking, only to need rescuing. She is brilliant in a fight, but she really doesn't think through anything. Lorcan is amazing. I know he might be on the "bad" list, but his background is so interesting. Eislyn(Reyna's sister) is really so sweet, but calculating. I enjoyed her and Thane's dialogue. The author did an amazing job with the imagery in this book. Everything was so detailed it was easy to fall into the scene. I love unexpected twists and while part of the ending I expected, I wasn't expecting how it took place. All in all, I found it very entertaining and I am very invested in continuing this series.
Favorite quotes:
"The truth may be twisted but never false."
"Who was she if she was not the enemy of the Air Court? What was her purpose of she no longer has that?"
"In a war-torn land, love was always a lie."
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on March 4, 2020
★★★★★ 5
Great Read!!! Great story!!!
Format: Kindle
The series is long, but Ms. Wolfhart does a fantastic job of weaving this tale while bringing so much to the characters. Surprises and plot twists along the way to keep you intrigued. There is some graphic sex, but is no way the focal point. Grammar was excellent (a rare find with a lot of self publishers) with only a few noted errors. I rarely give 4 stars, let alone 5.
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Reviewed in the United States on April 30, 2021
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