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Description
mIHC Dye (TSA540)Description mIHC Dye (TSA540) is a high performance fluorescent dye designed for multiplex immunohistochemistry (mIHC) based on tyramide signal amplification (TSA) technology. It enables robust, covalent fluorescent labeling on formalin fixed paraffin embedded (FFPE) tissues, frozen sections, and tissue microarray (TMA) slides, supporting multi target, in situ biomarker detection in a single tissue section. This reagent provides strong, stable
Description
mIHC Dye (TSA540) is a high-performance fluorescent dye designed for multiplex immunohistochemistry (mIHC) based on tyramide signal amplification (TSA) technology. It enables robust, covalent fluorescent labeling on formalin-fixed paraffin-embedded (FFPE) tissues, frozen sections, and tissue microarray (TMA) slides, supporting multi-target, in situ biomarker detection in a single tissue section.
This reagent provides strong, stable fluorescence with Ex/Em: 520 nm / 540 nm, compatible with standard fluorescence microscopes and multispectral imaging systems. It delivers greatly amplified signals compared to conventional immunofluorescence, ideal for low-abundance antigens and spatial profiling of the tissue microenvironment.
Key Features
TSA-based signal amplification: Ensures high sensitivity and high signal-to-noise ratio, suitable for detecting low-abundance antigens.
Covalent binding: Binds to tyrosine residues covalently, maintaining excellent stability during antibody stripping and repeated staining cycles.
Broad Compatibility: Applicable to FFPE tissues, frozen sections, and TMA slides, meeting diverse experimental needs.
Spectral Characteristics: 540 nm spectral channel, matching AF514, Opal 540, and similar orange-yellow fluorophores.
Easy to Use: Ready-to-use formulation with included signal amplification reaction buffer, no complex pre-processing required.
Application Scope: For research use only; not for in vitro clinical diagnosis or human trials.
Principle
TSA540 adopts horseradish peroxidase (HRP)-conjugated secondary antibodies to activate fluorescently labeled tyramide substrates. The activated tyramide rapidly forms covalent bonds with tyrosine residues near the antigen-antibody complex, forming stable fluorescent deposits. After that, the antibodies can be stripped by heat repair without removing the dye signal, allowing sequential labeling of multiple targets on the same tissue section to achieve multiplex staining.
Storage & Stability
Storage Condition: Store protected from light at 2–8 °C; avoid direct exposure to strong light.
Shelf Life: Stable for 12 months from the date of receipt when stored according to the required conditions.
Composition
TSA Monochromatic Fluorescent Dye 540, Signal Amplification Reaction Buffer
Application
Mainly used for scientific research of immunohistochemical staining of tissues, paraffin sections, and TMA chips; not for in vitro clinical diagnosis or human trials.
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4.7 ★★★★★
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Product Reviews
★★★★★ 4
Pieces chewed off
Color: white & green
My Aussiedoodle loves it. I throw it down the hallway, it bounces crazy, and he chases it. The only complaint is that he can't chew much of the ball part. So now I have to have it put away and brought out for special playtime.
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Reviewed in the United States on April 9, 2026
★★★★★ 5
Great toys
My 3-year-old Maltese destroys stuffed toys in short order. These toys with no stuffing keep her busy for hours without the risk of ingesting a bunch of loose stuffing. She loves them and carries them around the house making them squeak. Very durable for a toy destroyer like my little girl.
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Reviewed in the United States on April 3, 2026
★★★★★ 5
Fantastic toys! Your pup will love them!
My dogs love beating the ever loving **** out of these trusty squeaky toys. They’re durable, they last a long time, and they give my dogs plenty of entertainment. There’s no stuffing, which means zero cleanup. And the fact that you get 5 toys is a great value! They’re also super cute. I actually feel bad watching my dogs chew them.
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Reviewed in the United States on April 28, 2025
★★★★★ 5
Dog loves these!
My dog absolutely loves these toys! They hold up pretty well, the squeakers dont last long, but I am actually glad for that because I can only take so much squeaking. He gets so excited when I take a new one out and he carries them around everywhere. These are his favorite toys, by far.
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Reviewed in the United States on May 9, 2026
★★★★★ 5
Good value
Considering my girls will destroy any toy in no time and how much dog toys cost. These hold up as well as most and are a good value and the dogs like them.
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Reviewed in the United States on February 13, 2026