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Description
mIHC Dye (TSA540)Description mIHC Dye (TSA540) is a high performance fluorescent dye designed for multiplex immunohistochemistry (mIHC) based on tyramide signal amplification (TSA) technology. It enables robust, covalent fluorescent labeling on formalin fixed paraffin embedded (FFPE) tissues, frozen sections, and tissue microarray (TMA) slides, supporting multi target, in situ biomarker detection in a single tissue section. This reagent provides strong, stable
Description
mIHC Dye (TSA540) is a high-performance fluorescent dye designed for multiplex immunohistochemistry (mIHC) based on tyramide signal amplification (TSA) technology. It enables robust, covalent fluorescent labeling on formalin-fixed paraffin-embedded (FFPE) tissues, frozen sections, and tissue microarray (TMA) slides, supporting multi-target, in situ biomarker detection in a single tissue section.
This reagent provides strong, stable fluorescence with Ex/Em: 520 nm / 540 nm, compatible with standard fluorescence microscopes and multispectral imaging systems. It delivers greatly amplified signals compared to conventional immunofluorescence, ideal for low-abundance antigens and spatial profiling of the tissue microenvironment.
Key Features
TSA-based signal amplification: Ensures high sensitivity and high signal-to-noise ratio, suitable for detecting low-abundance antigens.
Covalent binding: Binds to tyrosine residues covalently, maintaining excellent stability during antibody stripping and repeated staining cycles.
Broad Compatibility: Applicable to FFPE tissues, frozen sections, and TMA slides, meeting diverse experimental needs.
Spectral Characteristics: 540 nm spectral channel, matching AF514, Opal 540, and similar orange-yellow fluorophores.
Easy to Use: Ready-to-use formulation with included signal amplification reaction buffer, no complex pre-processing required.
Application Scope: For research use only; not for in vitro clinical diagnosis or human trials.
Principle
TSA540 adopts horseradish peroxidase (HRP)-conjugated secondary antibodies to activate fluorescently labeled tyramide substrates. The activated tyramide rapidly forms covalent bonds with tyrosine residues near the antigen-antibody complex, forming stable fluorescent deposits. After that, the antibodies can be stripped by heat repair without removing the dye signal, allowing sequential labeling of multiple targets on the same tissue section to achieve multiplex staining.
Storage & Stability
Storage Condition: Store protected from light at 2–8 °C; avoid direct exposure to strong light.
Shelf Life: Stable for 12 months from the date of receipt when stored according to the required conditions.
Composition
TSA Monochromatic Fluorescent Dye 540, Signal Amplification Reaction Buffer
Application
Mainly used for scientific research of immunohistochemical staining of tissues, paraffin sections, and TMA chips; not for in vitro clinical diagnosis or human trials.
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4.6 ★★★★★
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Product Reviews
★★★★★ 5
Good pillow protector
Size: Queen Size (Pack of 2)
Looks and feels very durable. Didn't notice and odor when opened or in use. Fits my Coop Home Goods pillow perfectly! Sleep quality is still good with these covers on.
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Reviewed in the United States on March 29, 2026
★★★★★ 4
Very nice but expensive
Size: Queen Size (Pack of 2)
I love these cases, they didn't let out as much air as I had hoped for out the sides so I only zip it up half way so my pillow stays squishy and moldable and not partially filled with air which changes the feel of your pillow. Other then that they are great, they are very soft and quite, there is no noise whatsoever and there is some thickness to it adding a little more softness to your pillow. I ended up buying a second set. I just wish it had a little more airflow through the sides so I didn't have to leave it unzipped, that kind of defeats the purpose. I also think they are a little more expensive then they should be.
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Reviewed in the United States on February 2, 2026
★★★★★ 5
Does not change the pillows effectiveness at all.
Size: King Size (Pack of 2)
As of right now, I have not noticed anything with these covers that would significantly alter the way the pillows feel. I'm greatful that they are not noisy as most other covers are. I also appreciate that they have very much the same feel as the covers that come on the pillows. One thing I can say so far with confidence is that even though this is an additional layer to my pillows, the original feeling, specially with the Eden cool+, has been the same in terms of coolness and breathability.
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Reviewed in the United States on April 2, 2026
★★★★★ 5
SUPER SOFT - GREAT QUALITY!
Size: Crescent Queen
This crescent pillow protector is more than I expected, as far as softness and just the overall quality of it. I wish my PILLOW was as nice as this protector! Definitely recommend.
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Reviewed in the United States on March 23, 2026
★★★★★ 5
Exactly as described
Size: Crescent Queen
This pillow cover is everything they said it would be. Comfortable, no crinkle sound. Fits the pillow like a glove. I highly recommend.
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Reviewed in the United States on April 26, 2026