CD30/TNFRSF8 Fc Chimera Protein, Mouse
SKU: 50353089028

CD30/TNFRSF8 Fc Chimera Protein, Mouse

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Description

CD30/TNFRSF8 Fc Chimera Protein, MouseProduct Specification Species Mouse Synonyms CD30; CD30KI 1; CD30L receptor; D1S166EKi 1; TNFRSF8 Accession Q60846 Amino Acid Sequence Phe19 Thr281, with C hIgG Fc Expression System HEK293 Molecular Weight 72 95kDa (Reducing) Purity 95% by SDS PAGE Endotoxin <0. 1EU g Physical Appearance Lyophilized Powder Storage Buffer PBS, 5% trehalose, PH7. 4 Reconstitution Reconstitute at 0. 1 1 mg ml according to the size in ultrapure water after rapid

Product Specification


Species Mouse
Synonyms CD30; CD30KI-1; CD30L receptor; D1S166EKi-1; TNFRSF8
Accession Q60846
Amino Acid Sequence Phe19-Thr281, with C-hIgG Fc
Expression System HEK293
Molecular Weight

72-95kDa (Reducing)

Purity >95% by SDS-PAGE
Endotoxin <0.1EU/μg
Physical Appearance Lyophilized Powder
Storage Buffer PBS, 5% trehalose, PH7.4
Reconstitution Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
Stability & Storage · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
Reference

1. Oflzoglu E. et al. (2009) Adv. Exp. Med. Biol. 647:174
2. Kennedy, M.K. et al. (2006) Immunology 118:143.
3.Tarkowski, M. (2003) Curr. Opin. Hematol. 10:267.

Background

CD30, also known as Ki-1 antigen and TNFRSF8, is a 120 kDa type I transmembrane glycoprotein belonging to the TNF receptor superfamily. Mature mouse CD30 consists of a 264 amino acid (aa) extracellular domain (ECD) with three cysteine-rich repeats, a 27 aa transmembrane segment, and a 190 aa cytoplasmic domain. In contrast, human CD30 includes an additional 90 aa in the ECD and contains six cysteine-rich repeats. CD30 is normally expressed on antigen-stimulated Th cells and B cells. However, it is upregulated in Hodgkin’s disease (on Reed-Sternberg cells), other lymphomas, chronic inflammation, and autoimmunity. CD30 binds to CD30 Ligand/TNFSF8 which is expressed on activated Th cells, monocytes, granulocytes and medullary thymic epithelial cells. In B cells, CD30 ligation promotes cellular proliferation and antibody production in addition to the expression of CXCR4, CCL3,and CCL5. An 85 ‑ 90 kDa soluble form of CD30 is shed from the cell surface by TACE-mediated cleavage. Soluble CD30 retains the ability to bind CD30 Ligand and functions as an inhibitor of normal CD30 signaling.

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SKU: 50353089028

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