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Description
Human S100A11 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. 3. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. 4. Cell Lysis Buffer: Gently wash adherent cells with ice-cold PBS, then trypsinize and collect cells by centrifugation at 1000×g for 5 minutes. Suspension cells can be collected directly by centrifugation. Wash collected cells three times with ice-cold PBS and resuspend in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freeze-thaw cycles or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and remove the supernatant for analysis. 5. Cell Culture Supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for analysis or store at -20°C or -80°C, avoiding repeated freeze-thaw cycles. 6. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with S100 Calcium Binding Protein A11 (S100A11) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of S100 Calcium Binding Protein A11 (S100A11) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human S100 Calcium Binding Protein A11 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | S100 calcium-binding protein A11 (S100A11) is a protein encoded by the S100A11 gene in humans. The protein encoded by this gene is a member of the S100 family and contains two EF-hand calcium-binding motifs. S100A11, also known as calgizzarin or 100C, is a small, acidic protein. It is located as a group on chromosome 1q21, along with all 13 members of the S100 family. This protein may play a role in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene are associated with tumor metastasis. High expression is found in many tissues, including the lung, pancreas, heart, placenta, and kidney, with lower expression in skeletal muscle, liver, and brain. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.15-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenate, cell lysate, cell culture supernatant and other biological fluids |
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4.6 ★★★★★
Based on 1369 reviews
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Product Reviews
★★★★★ 5
Soft keyboards ( updated)
The keys fell off very easily after only 2.5 months of use. It is a nice and smooth keyboard. I like it, but it didn't last even 3 months. I am trying to get it repaired by them since it was so recent that I got it- I will update this review later once I hear from the manufacturer.
This is an update to my original post. I am extremely impressed with the quality of customer service and how fast they addressed my concerns regarding the product and they honestly back up their product. Not only did they respond fast, but they also replace my keyboard. Not just fix it, replaced the whole thing and it is also backed by their manufacturer warranty. I’m so happy and love that I get to have my keyboard back to use for hopefully a lot longer this time. It truly is a comfort keyboard and I love how smooth the keys feel when typing for long periods of time. Thanks guys for the prompt response!
Today 3/27/2026 I received my brand new keyboard to replaced the one with the broken keys and I’m so happy to it back again and fully working thanks guys for the great customer service. Truly appreciate it.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on March 24, 2026
★★★★★ 5
Great keyboard at the right price
I bought this to replace my old Logi Ergo K860. I like the idea of curved keyboard. But the distance between my left and right hands on Ergo K860 was bothering me ever since I bought it 5 years ago. I would say I never got used to it. The Ergo started to act up one day with one key F not working properly. I tried to fix it and considered getting another one, then I saw this keyboard with a much better price. So I took the chance and went with this cheaper option. I like this one better than Ergo for the following reason:
1. The layout is more comfortable. Yes, it took me a while to get used to it like I would with any keyboard. I don't have to twist my wrists to reach any keys.
2. Keys are not too loud. It's not going to silent, but better than Ergo.
3. Battery life is great. I make a living by typing my keyboard. I only charged this keyboard once so far (4 months).
4. The price is great for what I got.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on September 7, 2025
★★★★★ 4
Good keyboard, some auxiliary keys are moved from where I was used to
I've only been using this for a day, so I can't speak about battery life. It is rechargeable, and it has an on-off switch for saving the battery when I'm not using it, so I don't know what more I could ask for on that front. Likes: the main keyboard is definitely ergonomic and feels comfortable; the key action is not silent, which I actually like, and the scissor action switches underneath the keys feels good to me; the keyboard is about 1.5" narrower than my previous keyboard (Logitech Ergo K860) because the number pad is right next to the regular part of the keyboard, and the usual keys in between have been rearranged. Most of these changes will just require the user to get used to the new positions. However, the Home, End, Pgup and Pgdn keys are now Function keys, piggybacking on the arrow keys, and that may cause more trouble for people who use those a lot (I don't, so I don't mind it). Dislikes: experience has shown me that dust and crud get into the space between the wrist pad and the rest of the keyboard. On my old keyboard, I could remove the wrist pad using some tiny screws in the back, and take the pad out for thorough cleaning. With this keyboard I won't be able to do that, so time will tell how aggravating that will be. Maybe I'll have to take it down to my shop and blow out the crud with the air compressor (joking, probably not a good idea). Overall, not quite 5 stars, but close. If it lasts better than the Logitech, I may come back and put in that last star.
Update: It has lasted about 20 months, and I'm going to have to replace it. I may buy another one, 20 months is pretty good for the amount of use I have gotten out of it, but I'm going to search to see if anything new and better is around. Symptoms seem to indicate that the rechargeable battery is not taking a charge any more. The keyboard works find when it is plugged in, but when I unplug it, after a few minutes it goes wacky.
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Reviewed in the United States on May 16, 2024
★★★★★ 1
Arrived Battery DOA - But then something unexpected happened.
This was a one star review because the unit arrived with a batter that would not hold charge for more than a minute. I am not changing the stars, because if you are like me, you read the one star reviews cause so many companies pay for fake 4 and 5 star reviews.
When I first posted this review I also initiated a return and just planned to move on to another brand. I was surprised when I got a personal email from the Arctek Service Team offering to send me a replacement free of charge. With nothing to lose I figured why not?
The new unit arrived and I put a full charge on it and put it to work. I chose this keyboard because it is the closest in layout to the original Microsoft ergo split keyboard, no longer in production, that I have used for years and truly enjoy. I am pleased to report that the battery is working fine and the keyboard performs flawlessly. No skipped strokes or stuck keys, no glitches with the proprietary wireless interface to the laptop (when I am using it on the desk with a large screen).
My hat is off to the Arctek service team and management. A company that cares enough about its reputation and customers to go to so much effort to take care of a customer with such a small dollar item sale is a company you can expect to rely on. From now on when I want electronic gear on Amazon, I am going to use the word Arctek in my search.
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Reviewed in the United States on April 9, 2026
★★★★★ 5
Incredible
1 year update: I have been using this keyboard daily at work for almost one year and my 5 star review stands. Upon receiving the keyboard I charged it before use, and since then I have not charged it at all and it is still somehow at 75% battery!! I turn it off at night when I’m done.
One of the function keys broke off in a way where I can’t reattach it, but I reached out to customer support and they sent me a free replacement keyboard. Great customer service and warranty!
Original review: I love this keyboard. I am a grant writer so I write and edit all day. It has really leveled up my typing experience and has made work much more comfortable. It puts my hands in a natural position so my wrists no longer bend at odd angles. I adapted to the split key design in minutes. The keys have an excellent feel and sound to them and the wrist pad feels like a soft cloud. I’ve had no connectivity issues and so far the battery has been stellar. It also has rubber grippers on the bottom so it stays firmly in place on my desk and doesn’t move around like my old keyboard did. I went for this one because I didn’t want to spend $100+ on a keyboard and I’m really impressed with the quality.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on September 6, 2024
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