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Description
Human SDF-1 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. 3. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. 4. Cell Lysis Buffer: Gently wash adherent cells with ice-cold PBS, then trypsinize and collect cells by centrifugation at 1000×g for 5 minutes. Suspension cells can be collected directly by centrifugation. Wash collected cells three times with ice-cold PBS and resuspend in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freeze-thaw cycles or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and remove the supernatant for analysis. 5. Cell Culture Supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for analysis or store at -20°C or -80°C, avoiding repeated freeze-thaw cycles. 6. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP enzyme conjugate are sequentially added to microwells pre-coated with a capture antibody against Stromal Cell Derived Factor 1 (SDF-1). After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the amount of Stromal Cell Derived Factor 1 (SDF-1) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Stromal Cell Derived Factor 1 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Stromal cell-derived factor 1 (SDF-1), also known as CXC motif chemokine 12 (CXCL12), is a chemokine protein encoded by the CXCL12 gene on chromosome 10. Stromal cell-derived factors 1-α and 1-β are small cytokines belonging to the chemokine family. Members of these proteins activate white blood cells, typically in response to proinflammatory stimuli such as lipopolysaccharide, TNF, or IL-1. Chemokines are characterized by the presence of four conserved cysteines forming two disulfide bonds. They can be divided into two subfamilies. In the CC subfamily, the cysteine residues are adjacent to each other. In the CXC subfamily, they are separated by an intermediate amino acid. The SDF1 protein belongs to the latter group. CXCL12 signaling has been observed in several cancers. The CXCL12 gene also harbors one of 27 SNPs associated with an increased risk of coronary artery disease. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.15-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenate, cell lysate, cell culture supernatant and other biological fluids |
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4.4 ★★★★★
Based on 1056 reviews
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Product Reviews
★★★★★ 5
The Best "Stock Up and Save" Find on the Internet! ⭐⭐⭐⭐⭐
Size: 7.5 Fl Oz (Pack of 6), Scent: Fresh, Size: 7.5 Fl Oz (Pack of 6), Scent: Fresh
If you are tired of overpaying for fancy hand soaps that run out in a week, this Amazon Basics 6-pack of Liquid Hand Soap is the ultimate household hack. It’s simple, effective, and honestly, a total steal.
Why this 6-pack is a total winner:
The "Original Fresh" Scent: It’s exactly what you want in a hand soap—clean, light, and crisp. It’s not overpowering or flowery; it just smells like a freshly cleaned house.
Gentle on Hands: I wash my hands a lot throughout the day, and this formula doesn’t leave my skin feeling dry or cracked. It lathers up perfectly and rinses off clean without that weird "filmy" feeling some cheap soaps have.
The Perfect Size: The 7.5 fl oz bottles are the ideal "goldilocks" size. They fit on every sink—from the tiny powder room to the cluttered kitchen counter—without taking up too much space.
Bulk Convenience: Buying the 6-pack means I’m not constantly adding soap to my grocery list. I keep one at every sink and have backups ready in the pantry. No more "emergency runs" to the store when a bottle runs dry!
The Bottom Line:
You don’t need to spend $5 a bottle for clean hands. This Amazon Basics soap does the job perfectly, smells great, and saves you a ton of money over time. It’s a boring purchase that makes life so much easier.
Final Verdict: 10/10. Great lather, great scent, and even better price. I’m officially a convert!
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Reviewed in the United States on March 17, 2026
★★★★★ 5
Good price
Size: 50 Fl Oz (Pack of 1), Scent: Gentle & Mild
Most Amazon basics items are of decent quality and this is no exception.
Nothing fancy just a decent hand soap with typical viscosity that will work in just about any dispenser including automatic dispensers.
Most importantly it does a good job cleaning the hands without leaving your hands all sticky and gooey after washing. Yeah. That really is a thing. I've used hand soaps that after rinsing and drying your left with "gheco hands" like you can pick up a penny with your knuckle.
No chemical or overpowering odor.
One odd thing. The original order came in a box that looked like the Amazon loader threw it into the back of the van from about 50 feet away .... The bottles cap was destroyed and the soap had leaked into the box. Amazon quickly replaced the item with a new bottle no charge and didn't expect me to return the damaged bottle of course. Thing is the second box came in the same condition. Looked like the punter for the Ravens drop kicked it from the 30 into the back of the truck. Luckily the bottle was undamaged this time.
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Reviewed in the United States on April 11, 2026
★★★★★ 5
A Reliable, Budget-Friendly Alternative to Name Brands
Size: 7.5 Fl Oz (Pack of 6), Scent: Fresh
If you are looking for a straightforward, effective hand soap without the "name brand" markup, the Amazon Basics Original Fresh Liquid Hand Soap is an excellent choice. It offers a classic cleaning experience that rivals more expensive competitors, making it a staple for busy households.
Performance and Value
Effective Cleansing: This soap creates a rich, creamy lather that easily washes away dirt and grime, leaving hands feeling thoroughly clean.
Moisturizing Formula: Despite its deep-cleaning power, it contains moisturizers to help keep your skin from drying out, even with frequent daily washing.
Bulk Convenience: The Pack of 6 (7.5 Fl Oz each) ensures you have a steady supply for every sink in your home, from the kitchen to the guest bathroom.
Key Features
Dermatologist Tested: The formula is tested for safety and is designed to be gentle on the skin.
Clean Ingredients: It is formulated without phthalates, parabens, or triclosan, giving you peace of mind about what you're using on your body.
Light Fragrance: The "Original Fresh" scent is subtle and clean, providing a pleasant washing experience without being overbearingly perfumed.
Final Verdict
The Amazon Basics Hand Soap is a "no-frills" winner. It does exactly what it's supposed to do—clean hands effectively while maintaining moisture—all while saving you money. It is the perfect decent alternative for anyone who wants quality results on a budget.
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Reviewed in the United States on December 29, 2025
★★★★★ 5
Great value for your money & smells fresh & cleans well & nice lather
Size: 50 Fl Oz (Pack of 1), Scent: Gentle & Mild
I love tits effectiveness to clean & you can also add a pump functionality on it or you can just refill smaller bottles that have a pump which you can also buy here on Amazon or refill your old liquid handsoaps with pump, because this cleans well, nice lather , moisturizing feel & great for sensitive skin, smells fresh. I buy this a lot for my personal use & office use. Great value for your money.
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Reviewed in the United States on April 30, 2026
★★★★★ 4
Cleans well and smells fresh, but bottle is hard to pour
Size: 50 Fl Oz (Pack of 1), Scent: Gentle & Mild
I’ve been using this hand soap refill for a few weeks now, and overall I’m very happy with it. The soap has a pleasant scent that smells clean without being too strong, and it leaves my hands feeling refreshed instead of dry. I also like that the refill size lasts a long time and saves money compared to buying smaller bottles repeatedly. It lathers nicely and works well for everyday use in the kitchen and bathroom. However, the large refill container can be a little awkward to pour without spilling if you’re not careful. Still, the quality of the soap itself is great, and I would definitely purchase it again.
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Reviewed in the United States on May 12, 2026