SKU: 97186239198

Furin/PCSK3 His Tag protein, Mouse

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Description

Furin/PCSK3 His Tag protein, MouseProduct Specification Species Mouse Synonyms FUR,PCSK3,SPC1,PACE Accession P23188 Amino Acid Sequence Gln25 Glu714 with His Tag at the C Terminus Expression System HEK293 Molecular Weight 72 95kDa (Reducing) Purity 85% by SDS PAGE Conjugation Unconjugated Tag His Tag Physical Appearance Lyophilized powder Storage Buffer PBS, PH7. 4, 5% trehalose Reconstitution Reconstitute at 0. 1 1 mg ml according to the size in ultrapure water after rapid

Product Specification


Species Mouse
Synonyms FUR,PCSK3,SPC1,PACE
Accession P23188
Amino Acid Sequence

Gln25-Glu714 with His Tag at the C-Terminus

Expression System HEK293
Molecular Weight

72-95kDa (Reducing)

Purity >85% by SDS-PAGE
Conjugation Unconjugated
Tag His Tag
Physical Appearance Lyophilized powder
Storage Buffer

PBS, PH7.4, 5% trehalose

Reconstitution

Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.

Stability & Storage

· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· Please do not repeated freeze-thaw cycles.

Reference

1.Fitzgerald K. Furin Protease: From SARS CoV-2 to Anthrax, Diabetes, and Hypertension. Perm J. 2020;24:20.187.
2.Dahms SO, Hardes K, Becker GL, Steinmetzer T, Brandstetter H, Than ME. X-ray structures of human furin in complex with competitive inhibitors. ACS Chem Biol. 2014 May 16;9(5):1113-8.

Background

Furin, also known as Paired Basic Amino Acid Cleaving Enzyme (PACE), is a key member of the subtilisin-like proprotein convertase family. Functioning as a calcium-dependent serine protease, its structure includes an N-terminal signal peptide, a prodomain, a catalytic domain, a P-domain responsible for maintaining enzymatic activity and calcium/pH sensitivity, a transmembrane region, and a cytoplasmic tail. Primarily localized within the Golgi apparatus/endosomal system, it activates a wide range of proprotein substrates—such as growth factors, receptors, extracellular matrix proteins, and pathogen proteins (e.g., envelope proteins of HIV and influenza virus)—by specifically cleaving after the Arg-X-(Arg/Lys)-Arg sequence motif. It plays a critical role in embryogenesis, peripheral immune tolerance, and cellular homeostasis. Dysregulation of its function is closely linked to tumor progression, neurodegenerative diseases, and various pathogen infections, making it a significant therapeutic target in clinical applications.

Protocol

Assay protocol
Principle: Measured by its ability to cleave the fluorogenic peptide substrate, Pyr-Arg-Thr-Lys-Arg-AMC.
Materials
Assay buffer: 25 mM Tris, 1 mM CaCl2, pH 9.0
Furin/PCSK3 His Tag protein, Mouse (UA011277)
Substrate: Pyr-Arg-Thr-Lys-Arg-AMC (abs45133051)
96 ELISA Removable Plate, Black, High binding (GENEVER, Catalog # GMO2-96H)
Plate Reader (PerkinElmer, excitation 380 nm and emission 460 nm)
Produce
Dilute Mouse Furin Protein to 2 µg/mL/4 µg/mL/8 µg/mL in Assay Buffer.
Dilute Substrate to 100 µM in Assay Buffer.
Load into a black well plate 50 µL of 2 µg/mL/4 µg/mL/8 µg/mL of Mouse Furin Protein, and start the reaction by adding 50 µL of 100 µM Substrate. Include a Substrate Blank containing 50 µL of Assay Buffer and 50 µL of 100 µM Substrate.
Read at excitation and emission wavelengths of 380 nm and 460 nm, respectively in kinetic mode for 10 minutes.
Calculate specific activity:
*Adjusted for Substrate Blank
**Derived using calibration standard 7-amino-4-Methyl Coumarin
Conversion Factor (pmol/RFU)
Produce
Buffer: Assay buffer
Calibration standard: standard 7-amino-4-Methyl Coumarin (Enzo, Catalog # BML-KI107-0001).
Add 100 µL Dilute Calibration standard, Standard curve 1500/750/375/188/94/47/24/12/6/3 pmol per well or blank (Assay buffer) per well.
Read at excitation and emission wavelengths of 380 nm and 460 nm, respectively in endpoint mode.
Linear Regression of 7-amino-4-Methyl Coumarin (pmol) (y)—RFU-Blank(x).
Note
Storage Conditions: After reconstitution, aliquot and store at -80°C. Please do not repeated freeze-thaw cycles.

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SKU: 97186239198

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Brittany
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★★★★★ 5
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Size: Body Pillow & Pillowcase, Color: White, Size: Body Pillow & Pillowcase, Color: White
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Reviewed in the United States on February 27, 2026
J
JCMobile
Pawtucket, US
★★★★★ 5
My Wife Keeps Trying to Steal it-Its THAT Good!
Size: Body Pillow & Pillowcase, Color: White
I ordered this for myself because for the past 15 years I have had back issues and my chiropractor told me sleeping with a body pillow would really help. I also hate being hot when I'm sleeping, so I was excited when I saw the cooling feature. I got to use this for a couple of days until I made the mistake of telling my wife to TRY it and now she won't give it back. It's so comfortable. The pillow length is 4.5 feet, which is nice, but I wish it was a bit longer so I could put my whole leg on it. The 20-inch width makes up for the shorter length though, it's perfect. It gives plenty of support to take pressure off my back, knees, and hips. I've also been using the pillow horizontally under my legs occasionally. I love that the body pillow is adjustable meaning you can add or remove the shredded memory foam to how firm you want it. It comes with a pillowcase that is really nice too. Once you use this body pillow, you will never be able to sleep without it!
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Reviewed in the United States on April 8, 2026
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Ashley
San Leandro, US
★★★★★ 5
Great pillow
I absolutely love this pillow as it is very comfortable. The pillow is not too hard but not too soft. It is very well made and gives good support.
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Reviewed in the United States on May 16, 2026
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stephen hall
Los Angeles, US
★★★★★ 5
Best pillow ever made!!
The Bedgear pillows are the best!!! Most comfortable pillow anyone could use!!
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Reviewed in the United States on May 26, 2026
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J. M. Stone
Lexington, US
★★★★★ 4
BEDGEAR Does It Again!
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Reviewed in the United States on May 17, 2025

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