Human CCL2/MCP-1 ELISA Kit
SKU: 8544273373

Human CCL2/MCP-1 ELISA Kit

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Description

Human CCL2/MCP-1 ELISA KitProduct Specification Description Detection Principle: The double antibody sandwich ELISA method was used in this experiment. The anti human ccl2 mcp 1 capture antibody is coated on the microplate. After incubation, ccl2 mcp 1 in the sample and standard will bind to the antibody fixed on the plate, and the free components will be washed away; Then biotinylated anti human ccl2 mcp 1 detection antibody was added for incubation. After washing to remove

Product Specification

Description

Detection Principle:

The double antibody sandwich ELISA method was used in this experiment. The anti human ccl2/mcp-1 capture antibody is coated on the microplate. After incubation, ccl2/mcp-1 in the sample and standard will bind to the antibody fixed on the plate, and the free components will be washed away; Then biotinylated anti human ccl2/mcp-1 detection antibody was added for incubation. After washing to remove unbound substances, streptavidin HRP was added for incubation. After washing, the chromogenic substrate was added to avoid light for color development. The solution color is directly proportional to the bound target protein; Add termination solution; The absorbance was measured with a microplate reader.

Detection Type: Double antibody sandwich method

Form: pre coated 96 well plate

Detection Sample Type: cell supernatant, serum, Plasma

Loading amount: 100ul

Kit Components:

Pre coated 96 well plates, standards, anti human   One copy of ccl2/mcp-1 detection antibody, dilution buffer, chromogenic solution (a, b), washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.

Sensitivity: 18.9  Pg/ml

Detection Range: 15.6 - 1000 pg/ml

Recovery Range: 85-104%

Storage Method: 2-8 ℃

Standard Curve 

Background:

CCL2/MCP-1 is a chemokine that binds to the receptor CCR2 and induces chemoattraction of monocytes. It induces the activation of monocytes, NK cells, lymphocytes, and basophils. In addition, CCL2 promotes Th2 polarization of cd4+ T cells, and CCL2 mediated recruitment of monocytes to inflammatory sites exacerbates atherosclerosisSeverity of sclerosis, multiple sclerosis, and allergic asthma. Endogenous proteolytically modified CCL2, including N-terminal pyrrolidone carboxylic acid modified glutamine, downregulated activity but did not bind to the receptor.

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SKU: 8544273373

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Louisville, US
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Hydrating
Size: 10.14 Fl Oz (Pack of 1)
very hydrating for everyday use, no fragrance, great overall cream for dry skin, haven't seen much change in lightening my skin tone if that's what you're looking for
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Best body lotion
Size: 10.14 Fl Oz (Pack of 1)
Best body lotion
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Tigerlily b.
Natrona Heights, US
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It's an okay lotion
Size: 10.14 Fl Oz (Pack of 1), Size: 10.14 Fl Oz (Pack of 1)
It can as advertised, it has no scent but it makes my skin smooth and soft but it's not moisturizing so it's best to use an oil after. It actually tighten my skin over time.
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SuzyQ 2032
Battle Creek, US
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This product is the real deal.
OMG. This is the best skin care product I have ever used. I'm 61 years old and my skin was showing sun damage, dark circles, real wrinkles around my eyes, lips, and forehead. Basically, I was looking every bit of my 61 years. About 30 days ago I started using this organic beef tallow with raw honey moisturizer in conjunction with a product to clear the pores on my nose. (That's a different review.) I will never go back to the 5, costly products I was using. I was using a daytime moisturizer with SPF, a dark circle cream, a dark spot remover, a fine line product, and a night time resurfacing cream before trying this product. That was a couple hundred dollars every 5 weeks or so. Since starting this beef tallow and raw honey 30 days ago, my skin looks 10 times better. It's dewy but not greasy. My makeup looks good ALL day. My wrinkles are less noticeable and, I think, reduced in depth. My under eyes look so good. No more concealer under my foundation. In fact, I can go out and about with no makeup now! This product glides on easily, no dragging or pulling. It never feels greasy. And it only takes about a marble sized amount for my full face, neck, and decolletage. I use it morning and at night. So far, the little jar is still 3/4 full. Amazing product. I will be buying more for sure. Oh, one more thing. About 2.5 years ago, I had to have a mass removed from the left side of my neck. The scar was very noticeable. This cream is even improving that! Try it. See if I'm wrong!
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Yaya
Louisville, US
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I am honestly blown away by this product. My skin feels so soft. I also feel as a mature lady that my lines have softened. My daughter burned her hand very badly and we were using a prescription cream for 2 weeks with little improvement. I used this and seen huge improvement in just days. It doesn't feel heavy or greasy and absorbs nicely. The smell is mild and pleasant. My teenage son has started using it for skin irritation and just 2 days and he's impressed. The jar has seemed to go a long way especially for 3 ppl using it. 10/10 will buy again. I am not paid for this and am a real human.
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