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Description
Mouse JAML ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. Cell Lysis Buffer: Gently wash adherent cells with ice-cold PBS, then trypsinize and collect cells by centrifugation at 1000×g for 5 minutes. Suspension cells can be collected directly by centrifugation. Wash collected cells three times with ice-cold PBS and resuspend in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freeze-thaw cycles or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and remove the supernatant for analysis. Cell Culture Supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for analysis or store at -20°C or -80°C, avoiding repeated freeze-thaw cycles. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a junctional adhesion molecule-like (JAML) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the amount of junctional adhesion molecule-like (JAML) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse Junctional adhesion molecule-like ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Junctional adhesion molecule-like protein (JAML), or AMICA1, is a member of the JAM family of transmembrane proteins. It consists of two extracellular immunoglobulin-like domains, a transmembrane region, and a cytoplasmic tail involved in activation signaling. Known JAML ligands are coxsackievirus and adenovirus receptors, and it has been shown to localize to tight junctions on epithelial cells. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenate, cell lysate, cell culture supernatant and other biological fluids |
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4.1 ★★★★★
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Product Reviews
★★★★★ 5
My favorite body wash
Scent: Bergamot, Size: 16 Fl Oz (Pack of 1), Scent: Bergamot, Size: 16 Fl Oz (Pack of 1)
My go to body wash, you just get so much for the price and it lasts forever! The smell itself is also very relaxing after a long day. It also lathers quite nicely and does it's job well, would definitely recommend
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Reviewed in the United States on April 13, 2026
★★★★★ 5
Smells Like You Have Your Life Together
Scent: Bergamot, Size: 16 Fl Oz (Pack of 1)
Cremo Body Wash with Italian Bergamot, Neroli Blossom, and Fresh Vetiver smells far more expensive than it has any right to. The scent hits that sweet spot between fresh and refined. The bergamot gives it a clean citrus lift, the neroli adds a smooth floral edge, and the vetiver grounds it with a subtle, masculine depth that does not scream but absolutely speaks.
The lather is rich and concentrated, so you only need a small amount. It feels more like something you would find in a boutique hotel than on a regular store shelf. The fragrance lingers just enough to be noticed without overpowering your cologne or filling the entire room.
If you want to step out of the shower smelling like competence and quiet confidence, this one delivers. It is polished without trying too hard, and it makes everyday showers feel slightly upgraded.
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Reviewed in the United States on February 28, 2026
★★★★★ 5
Profound and timely
Format: Paperback
If I could give this book 6 stars I would. The way the world is right now, I needed to do a deep dive into anger. And I needed to see how God wants me to handle my anger. There were some very challenging and humbling parts to this book that I needed to hear. All the application questions at the end of each chapter helped me solidify what I learned and what I needed to evaluate personally. Which leads me to the statement that this book was deeply personal to me and I am grateful for the changes it’s helped me make to reflect God more and myself less.
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Reviewed in the United States on March 26, 2026
★★★★★ 5
Profoundly biblical and wise
Format: Paperback
David Powlison’s Good and Angry is a powerful book. In the book Powlison dives deep into what anger is and then gets very practical about how to biblically deal with your own anger issues (issues, he assures us, we all have).
From the outset, Powlison makes it clear that anger, while dangerous, can be handled to produce good. “At its core anger is very simple,” Powlison says, “It expresses ‘I’m against that’” (39). Powlison says that each of us handles anger differently. Some of us freeze over, some of us quietly brood, some of us simmer, some of us explode. Powlison encourages us not to look at the way others mismanage worse than us, but rather, how do we mismanage anger? Each type has their own blind spots.
Powlison then dives into anger itself. Anger is about our displeasure toward something, so what are we displeased with? And why? How are we justified? Unjustified in our anger? And what do I want to happen? Anger is physiological. As embodied beings, anger manifests itself in us physiologically. How is it impacting me when I’m angry?
Powlison then dives into mercy, what he calls a constructive displeasure, or constructive anger. When the constructive displeasure of mercy is functioning as it ought, it has four characteristics: patience (a wonderful biblical synonym of patience is “forbearance”), forgiveness (which is “mercifully unfair” (80)), charity (a spirit of magnanimity), and constructive conflict (“Mercy is not a free pass. It is an invitation to turn and repent” (94). All of these fundamentally point to the work of God and his righteous response of anger to our rebellion. “The constructive displeasure of mercy means the redemption of the world” (102). Powlison walks through how God’s anger works: through his righteous and holy response to our sin, to him taking his wrath upon his son on the cross. He concludes, “God’s wrath is your hope. God’s wrath is my hope. We don’t often hear that, but it appears everywhere in the Bible. Wrath is our hope because love masters anger” (121).
The final portion of the book steps back and helps us move through analyzing our own anger. Powlison uses James 4:1-12 to help us analyze our own anger issues. At the heart of this analysis is James’s own analysis of his hearer, that they are fighting and quarrelling because of their “desires that battle within” them. In other words, if we have an anger problem (which we all do), we have a malformed desire problem. In other words, we have a heart problem. Significant in digging into this question is the ability to analyze my own motives. The issue isn’t what has happened with me, but is my heart and my heart’s motives and desires in the midst of any given situation. Key questions to ask myself when in a moment of anger are: “what do I want?” “what do I fear?” and “what do I most love?” (154-55).
Powlison concludes with a strong word of hope. God is in the process of changing us and reshaping our heart. Our problem, Powlison says, is that we tend to talk to the wrong person in the midst of our anger – ourselves. But when we turn and talk to our Good Shepherd, we will experience hope and change.
I’m so grateful for Powlison's Good and Angry. It is a profoundly biblical and wise book with both subtle and profound insights. I know I have been impacted by the book personally and will both turn to it in the future for personal use and as a resource for others who struggle with anger.
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Reviewed in the United States on April 21, 2017
★★★★★ 5
But he is also convinced that your anger does not have to be a problem and this book is intended to help redirect so that we can
Format: Paperback
David Powlison thinks you have an anger problem, and a serious one at that. It is not because he knows you personally, but because he is one who has long studied the fallen heart. But he is also convinced that your anger does not have to be a problem and this book is intended to help redirect so that we can be both good and angry.
Anger, as Powlison who is the executive directory of CCEF, defines it, is a sense of opposition to something that is both important and wrong. That means it is a moral response. As those who are created in the image of God we all have come wired for the capacity to express anger. But, as Powlison carefully shows, we have a problem. Our anger is misdirected and misguided because of sin. Not only in the little daily frustrations and irritations, but also in extremely destructive ways. The goal, according to Powlison, is not to eliminate anger, but to have it remade into the image of God by the grace of the gospel. Far from being a therapeutic self-help book, his concern is that we come to understand how anger can coexist with forgiveness, patience, charity, and constructive displeasure.
This book should prove to be an excellent resource. However, if you pick it up with the intention to help someone else—your husband, wife, child, friend, or congregant—be prepared to confront the reality of your own anger first. Powlison has a wonderful way of acutely diagnosing anger as a personal problem for all, even those who do not consider themselves angry. A couple of highlights would be his detailed examination of God's wrath showing readers why it is actually because of God's wrath that we have hope. His chapters dealing with every day anger, long-held bitterness, and anger against God also flow from a counselor's heart as he addresses difficult and awful painful truths.
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Reviewed in the United States on September 23, 2016