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Description
Human SOD ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. Cell Lysis Buffer: Gently wash adherent cells with ice-cold PBS, then trypsinize and collect cells by centrifugation at 1000×g for 5 minutes. Suspension cells can be collected directly by centrifugation. Wash collected cells three times with ice-cold PBS and resuspend in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freeze-thaw cycles or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and remove the supernatant for analysis. Cell Culture Supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for analysis or store at -20°C or -80°C, avoiding repeated freeze-thaw cycles. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP enzyme conjugate are sequentially added to microwells pre-coated with a superoxide dismutase (SOD) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of superoxide dismutase (SOD) in the sample. The absorbance (OD value) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Superoxide Dismutase（SOD) ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Superoxide dismutase (SOD) is an enzyme that catalyzes the conversion of superoxide into oxygen and hydrogen peroxide through a dismutation reaction. It is widely found in various animals, plants, and microorganisms and serves as an important antioxidant, protecting cells exposed to oxygen. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.312-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenate, cell lysate, cell culture supernatant and other biological fluids |
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4.6 ★★★★★
Based on 1132 reviews
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Product Reviews
★★★★★ 5
A story of art, music and writing
Format: Kindle
Like the face of heaven. That’s how I see Theo, and I hope this will be a movie.
“Theo of Golden” is the debut novel by Allen Levi. He was writing as a personal challenge and just doing some scenes. He began writing in 2019-2020. He had been a songwriter and lawyer. He has no connection to Portugal, which is mentioned in the book quite a bit and led me to try Port wine. This book will lead me to do a lot of things.
Theo is a supremely interesting man! More on that other familiar interesting man in a later post.
The book will make you want to look at the faces of people. Use a gaze that calms and loves. Be kind and generous. Greet people. Be less fearful and exchange stories. Be attentive. Love people up close. Invest in the art of living. Be beautiful, useful and pleasurable. All which I should be doing, but will now do for Lent.
Theo listened with genuine interest, adoringly, encouraging and with gratitude.
Newborns look for their mother’s face, a particular gaze that calms, loves and welcomes them. Someone they recognize, run to greet and someone to bond with.
Levi signs the books “With thanks for the Theo in you.” He greets kids at a high school three days a week. He reads to second graders. He doesn’t mind being around people not like him.
The coffee shop setting is a real place in Columbus, Ga. It’s called Fountain City Coffee. In picking bestowals of the sketches there, Theo looked for someone lost, weary, worried or troubled that the gift would encourage or help.
I got to hear him in a Zoom. A group of women from Vermont who lost their daughters came to Georgia on a pilgrimage. He was a steward of clean rivers and water and said the river still flows after death. He went through a “walking season” in losing his daughter.
In a book about knowing a person, it is mentioned that a bench is a good way to do this. 7 p.m. was Theo’s meeting time with the strangers for bestowals and ended up being the time of the funeral. He also mentioned “Think Little” by Wendell Berry. Do so and if everyone does, it spreads.
Levi was born in 1956. His dad is 98. His mother passed and he lost his brother, whom he called a clone of Theo, at 55. His dad was a forester, explaining his interest in nature. Springtime is an end with a future, said Theo.
One character named Tony runs the Verbivore book store and has the Penny Loafers out there because they haven’t spent a penny and they majored in loafing. Theo and Tony end up having Port together and he told Theo is Viet Nam stories. The Penny Loafers were better than a room full of Senators. They lie. Tony ends up being more of a believer of Jesus at the end because he wasn’t before Theo.
James Ponder has no degrees hanging in his office. Note the “ponder” mentions throughout. He is a dispenser of advice, secret keeper and agent of calm. Secretary Mrs. Gidley was suspicious of Theo at first but comes around. Ponder was Theo’s landlord and mentioned the 18-inch railing and three flights of stairs before the 86 year old decided to live there.
Kendrick reminds me of the custodian who won “America’s Got Talent,” who has an ill daughter Lamisha, who wants to be a physical therapist or artist in the end. Her education will be paid for by Theo as were some of her medical bills. Maria Menendez is the daughter of the “little man” who killed Theo’s wife and daughter in an accident. Maria had cancer.
I don’t want to give away too much, but Theo decided to love Asher, the sketch artist, with two hearts. And there is much more to that.
Simone wears a wardrobe that honors music on stage and guests who come to listen did the same. Cello intricacies became fascinating to me and I looked up some of the music mentioned. His bow was a gift from Theo. He played Fado for Theo at his concert. It ended up being a requiem with three characters, Kendrick and Basil, the busker. Simone’s parents were there and I wasn’t clear if Theo sprang for their trip. At the end, Basil studies to get a doctorate in American literature.
Katherine is the reporter who respects Theo’s wishes while many people she interviews are praise seekers and applause junkies. He was a friend, not a headline.
Ellen rides a bike and I’ll just call her different. He gave her a foldable handsaw, cordless sander, drill and wood burner to make feather boxes. I have made one because of this book. She had $1,600 to her name from selling them, but gave the money to the cello fund after she and Simone were assaulted and the perpetrator was never caught.
Cleave is the poisonous guy—like a snake, hemlock, black widow or “words with no wisdom guy.”
All but Cleve show community in sitting on the same row or pew. They all have sparks of hope.
I loved this line: We walk all roads, long and winding. Road to ruin. Easy Street. Road less traveled.
I’m so mad that my notes from Chapter 10-28 somehow disappeared. I may reread. This post would be longer. I probably have a dozen favorite books and just added this one.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on February 5, 2026
★★★★★ 5
Recognizing the Hearts that Burn Within Us
Format: Paperback
Yes, Theo of Golden does, at times, border on the melodramatic and the sentimental. It can be repetitive. It can take an awfully long time to make its point. And yet, the novel speaks very significantly of the need for love, compassion, generosity and forgiveness---in a world that is not always kind, in a world where many of us carry secret burdens---where sadness (mixed with intermittent joy) might be viewed as a universal human affliction.
Theo of Golden is, overall, a book of glad tidings. An end with a future. Thoughtfully, warmly and intelligently written. A novel that often addresses the beauty of Art, most notably painting and music. A novel in which I learned what a fado is. In fact, I learned a lot from this novel.
Unlike some readers, I did not see the book as misogynistic or xenophobic. I didn't necessarily see it as "Christian fiction"---unless you mean doing good, striving for strength of spirit and understanding the connection between "the ultimate and the proximate, the wide grace and the narrow way."
Finally, I don't view the novel's conclusion as disappointing, but simply as falling action and resolution. It is, after all, a very traditionally structured work.
Kudos to the author Allen Levi for having written an initially self-published book that was later picked up by a major publishing house. That is, indeed, a rarity.
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Reviewed in the United States on May 17, 2026
★★★★★ 5
better than the series
Format: Kindle
The chemistry the characters the banter the amazing direction of the story all builds up to a crescendo that simple didn’t let go! This is the first of an epic series that simply let love in its many different ways and journey transformed lives in ways that one couldn’t imagine! Beautifully worded and crafted with a creative nack to entertain and really dig this universe! Well done Miss Kennedy, well done!
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Reviewed in the United States on May 29, 2026
★★★★★ 4
Not forced or rushed
Format: Hardcover
I really liked this book. Hannah & Garrett’s story starts off like a typical smart girl tutors the jock to help him get though a class in college. But their stories are traumatic and the things that they have both had to overcome make this book so much more than your typical tutor/jock story. Garrett is sweet, cocky, funny and helps bring Hannah out of her shell in a refreshing way. Hannah is not meek or mild or quiet, she is strong because of her past but still a little unsure. Together their chemistry is hot and their relationship just sort of naturally unfolds. I really loved that it didn’t seem forced or rushed, it just happened as it should. Garrett’s story in particular hit very close for me so maybe that is why I felt connected to these characters so much. I am really looking forward to reading the rest of the series.
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Reviewed in the United States on May 21, 2026
★★★★★ 5
Hot, Sweet, and Real
Format: Kindle
First I love a hockey romance! Add in fake dating, he falls first, and shared trauma and you have me hooked! This has been on my TBR longer than I want to admit! With the show that came out I wanted to read this first and it did not disappoint! I loved it! Garret and Hannah together crack me up and are so cute! This book had sad, funny, serious, and spicy moments! Highly recommend! The audiobook voice actors did a good job! I would recommend reading the trigger warning page this book does talk about some issues/trama that can be triggering.
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Reviewed in the United States on May 19, 2026