UniOne® TR-FRET Human IL6/IL6R Binding Kit
SKU: 47361711801

UniOne® TR-FRET Human IL6/IL6R Binding Kit

Sale price$328.50 Regular price$365.00
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Description

UniOne® TR-FRET Human IL6/IL6R Binding KitProduct Specification Host Human Stability & Storage 80 Background The assay kit employs Homogeneous Time Resolved Fluorescence (TR FRET) technology to measure the interaction between IL 6 and IL 6R. This method enables simple, rapid, and high throughput identification of inhibitors and antibody based blockers. As illustrated, the interaction between IL 6 and IL 6R is detected using a Eu labeled anti Tag1 antibody (TR FRET donor) and an Acceptor

Product Specification


Host Human
Stability & Storage

-80℃

Background

The assay kit employs Homogeneous Time-Resolved Fluorescence (TR-FRET) technology to measure the interaction between IL-6 and IL-6R. This method enables simple, rapid, and high-throughput identification of inhibitors and antibody-based blockers. As illustrated, the interaction between IL-6 and IL-6R is detected using a Eu-labeled anti-Tag1 antibody (TR-FRET donor) and an Acceptor-labeled anti-Tag2 antibody (TR-FRET acceptor). Upon binding of IL-6 to IL-6R, the donor and acceptor antibodies are brought into close proximity. Excitation of the donor antibody then triggers Fluorescence Resonance Energy Transfer (FRET) to the acceptor antibody, resulting in specific emission of a 665 nm wavelength signal from the acceptor. The intensity of this specific signal is proportional to the extent of IL-6/IL-6R interaction. This homogeneous assay features a straightforward workflow with no washing steps required.


Components

Table 1. Reagents and Storage Conditions

Component

Concentration

500 TESTS

2500 TESTS

10000 TESTS

Storage Temp.

Tag1-IL6 protein

100×

20μL

100μL

400μL

-80°C

Tag2-IL-6R protein

100×

20μL

100μL

400μL

-80°C

Tocilizumab

10 μM

10 μL

50 μL

200 μL

-80°C

Anti-Tag1 Eu cryptate antibody

50×

50μL

250μL

1000μL

-80°C

Anti-Tag2 Acceptor antibody

12.5×

200μL

1000μL

4000μL

-80°C

Detection buffer

10×

2mL

10mL

40mL

-80°C

* Avoid repeated freeze-thaw cycles of reagents. Aliquot upon first th

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Exchange/Return Notes
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SKU: 47361711801

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LSch01
New York, US
★★★★★ 3
Assembly is harder than it should be; you get what you paid for
Color: Black, Size: 4 Panel-88''
Review of SUNALLY Room Divider 6FT Folding Privacy Screens, 4 Panel Fabric Divider for Room Separation, 88" W Freestanding Portable Wall Dividers Screen for Home Dorm Studio Office, Black I hate when I have to leave products a not so good review... Assembly is harder than it should be, mainly because there shouldn't BE any assembly. I have ordered several room divider privacy screens over the last couple of years and not one of them needed any kind of assembly. With that aside, it STILL was a pain in the sphincter to assemble. One of the "legs" just sits on top of a foot plate - it's not screwed in to anything. It's kind of flimsy and the base plates don't have anything on them to not scuff up the floor. With all that said, though, it does stand up and will block out what I need to block out. At the less-than-$50 price-point, it's kind of what should be expected. If I had purchased this outright, I would have considered sending it back and getting a higher quality. But if you only have $50 to spare and don't mind assembling things, it could be good for you.
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Reviewed in the United States on April 28, 2026
O
Ocean
Belleville, US
★★★★★ 5
Great buy
Color: Black, Size: 4 Panel-88''
This room divider is exactly what I needed. When you have 2 kids sharing a room and don’t want to spend a lot of money to divide their space this is what you can use. Believe it or not they are much happier now. This came in a small box which scared me but once I was able to get this up I was very pleased. It was easy to put together. The fabric is pretty good quality, it’s stable and stands up fine. Good value and worth it to have peace and quiet in the house.
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Reviewed in the United States on May 18, 2026
B
Verified Purchase
Beth Lukes
Omaha, US
★★★★★ 1
Junk
Color: Black, Size: 4 Panel-88'', Color: Black, Size: 4 Panel-88''
Horrible. The parts are not right. It won’t fit together or disassemble and can’t return it like this. Parts won’t fit properly and missing holes for screws. Also, can’t get through to a person for any assistance. Picture shows the hole and then the lack of a hole where a screw belongs.
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Reviewed in the United States on May 13, 2026
B
Verified Purchase
Ben F.
Alexandria, US
★★★★★ 5
Good product, poor directions.
Color: Black, Size: B-88''W-4 Panel
The quality of the product was better than most at this price. The directions could have been much better even though I have an engineering background.
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Reviewed in the United States on September 1, 2025
D
Verified Purchase
Deb J
Battle Creek, US
★★★★★ 4
Nice partition for my basement
Color: Beige, Size: B-102''-3 Panel
This 3-panel room divider showed up well-packaged with all the parts and even a little tool for putting it together. The instructions were easy to follow, and while one person can definitely assemble it, having two makes it quicker and less frustrating. It does exactly what I needed—gives quick, easy coverage in an open area. I'm using it in my basement to hide the furnace and water heater, and it works great for that. It looks nice overall, but I picked the beige fabric (which is really more of a tan), and the black frame makes it stand out more than I’d like. A white or lighter frame would’ve blended better. Not sure that I would use it anywhere besides the basement It’s lightweight and pretty sturdy. The wheels make it easy to move, but it does get a bit wobbly, and the panel clips need to be readjusted every time you roll it somewhere new. Still, for the price, it’s a solid buy. I’m happy with it and planning to order another one.
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Reviewed in the United States on June 21, 2025

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