Recombinant Carboxypeptidase B,Bovine
SKU: 30258998695

Recombinant Carboxypeptidase B,Bovine

Sale price$131.40 Regular price$146.00
Save 10%

Shipping Estimate
USA
  • USA
  • CAN

Ships within 48 hours · Estimated delivery Jul 7 - Jul 12

Promo Codes Available:

For Your Every Summer RSVP, with Code: SUMMER15

Description

Recombinant Carboxypeptidase B,BovineProduct Specification Species Bovine Synonyms Carboxypeptidase B Expression System PichiaPastoris Molecular Weight 33 kD Purity 95% by SDS PAGE & HPLC Tag His Tag Physical Appearance Lyophilized Powder Storage Buffer 20mM Tris HCl 100mM NaCl Reconstitution Carboxypeptidase B lyophilized powder was dissolved in sterile water or 25mM Tris HCl (pH7. 6) to make the concentration of enzyme solution 1 10mg ml. Stability & Storage 24 month, 2 to 8 C under

Product Specification


Species Bovine
Synonyms Carboxypeptidase B
Expression System PichiaPastoris
Molecular Weight

33 kD

Purity >95% by SDS-PAGE & HPLC
Tag His Tag
Physical Appearance Lyophilized Powder
Storage Buffer 20mM Tris-HCl 、100mM NaCl
Reconstitution

Carboxypeptidase B lyophilized powder was dissolved in sterile water or 25mM Tris-HCl (pH7.6) to make the concentration of enzyme solution 1-10mg /ml.

Stability & Storage

·24 month, 2 to 8 °C under sterile conditions
. After dissolution, store at -20℃ and avoid repeated freeze-thaw cycles.

Reference

[1] Akparov V, Sokolenko N, Timofeev V, Kuranova I. Structure of the complex of carboxypeptidase B and N-sulfamoyl-L-arginine. Acta Crystallogr F Struct Biol Commun. 2015 Oct;71(Pt 10):1335-40.
[2] James W. Brodrick, Michael C. Geokas, Corey Largman, Human carboxypeptidase B. II. Purification of the enzyme from pancreatic tissue and comparison with the enzymes present in pancreatic secretion, Biochimica et Biophysica Acta (BBA) - Enzymology, 1976, 468-481.

Background

Carboxypeptidase B, also known as peptidyl-L-lysine (L-arginine) hydrolase, contains one Zn atom per molecule and can selectively hydrolyze arginine and lysine at the carboxyl end of proteins or polypeptides. CPB can be used in the production of recombinant insulin and its analogues, and the quality detection of recombinant antibodies. and also used in the production of other recombinant polypeptides.

Components

20mM Tris-HCl 、100mM NaCl、Mannitol、Carboxypeptidase B

Protocol

The recommended amount of Carboxypeptidase B is 1:50~1:1000 (w/w) compared with the target protein (mass ratio), the optimal pH is 7.5-9.0, and the appropriate enzyme digestion temperature is 25℃~37℃.

Guidelines

1. For different protein samples, it is necessary to explore the optimal enzyme concentration and reaction time.

2. Reaction at 25℃~37℃, the reaction time can be appropriately extended when digestion at 25℃.

Unit Definition

The amount of enzyme that catalyzes the hydrolysis of 1 μmol of maroyl-L-arginine in 1 min is one enzyme activity unit of carboxypeptidase B.
Shipping Notes
  • Free Standard Shipping on $100+ Orders to the USA.
  • Except Preorder products are shipped in 48 hours.
  • Delivery to the USA:
  1. Standard Shipping : 3-10 business days
  • If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
  • We offer a 30-day return/exchange service after receiving.
  • Final sale items are not eligible for returns or exchanges.
  • To process your return/exchange, please contact us at [email protected]
  • Please click here for more details>>> Return & Exchange Policy
SKU: 30258998695

Discover Niche Categories That Outsell

Top-Converting Item to Boost Your Average Order

4.4 ★★★★★
Based on 2137 reviews
Sort
Highest Rating
Newest First
Oldest First
Product Reviews
A
Verified Purchase
Ahsaki Bailey
West Palm Beach, US
★★★★★ 5
Men turtleneck
Size: Medium, Color: A-black
its soft, warm fabric, true-to-size fit, would suggest sizing up for person with muscles to avoid too tightly of a fit.🥰
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on March 3, 2026
D
Verified Purchase
Darmea
Lexington, US
★★★★★ 5
Turtle-Turtle!
Size: X-Large, Color: A-black
Fits well, wears well, and holds up well in the washer/dryer. This is a nice product and has not stretched, faded or shrank. The neck part of this turtle comes up to just under the chin (folded over) and stays snugly in place when pulled over the head. The slim fit is nice for me, but may not work with beefier bodies. Arms are not baggy but fit well. Sleeves are snug on the wrist. It keeps you warm but is not bulky. Fits nicely under sport coats, outer jackets, and warm up suit jackets. I'm very satisfied with this turtle and recommend it.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on November 11, 2024
S
Verified Purchase
Shon Brown
Houston, US
★★★★★ 5
Turtle neck sweater
Size: Large, Color: Wine Red, Size: Large, Color: Wine Red
This turtle sweater is spot on and feels so soft. The quality of material is top notch and the color is amazing on me. You can’t go wrong with this one fellas.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on February 17, 2026
A
Verified Purchase
Angie B
Draper, US
★★★★★ 3
Cheap but stylish :) I still like
Cute, weird shoulders, cheap. Will tear fast, and earth. 1 thread and it’s overreeerrrrrr
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on February 8, 2026
C
Verified Purchase
CHTHEV
Charlottesville, US
★★★★★ 5
Pleasant surprise
Size: Small, Color: Wine Red
Good quality. Love how the neck fits. The fabric is soft and comfortable. Size fits are accurate. I recommend this product. We purchased it twice in different colors. Worth the money!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on December 14, 2025

recommand products