SKU: 27148892891

Rat TSH ELISA Kit

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Description

Rat TSH ELISA KitProduct Specification Usage Sample Processing and Requirements 1. Serum: Place the whole blood specimen collected in a serum separator tube at room temperature for 2 hours or at 4C overnight, then centrifuge at 1000g for 20 minutes. Remove the supernatant and store it at 20C or 80C. However, repeated freezing and thawing should be avoided. 2. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Within 30 minutes of collection,

Product Specification

Usage

Sample Processing and Requirements

1.
Serum: Place the whole blood specimen collected in a serum separator tube at room temperature for 2 hours or at 4°C overnight, then centrifuge at 1000×g for 20 minutes.
Remove the supernatant and store it at -20°C or -80°C.
However, repeated freezing and thawing should be avoided.

2.
Plasma: Collect the specimen using EDTA or heparin as an anticoagulant.
Within 30 minutes of collection, centrifuge at 1000×g for 15 minutes at 2-8°C.
Remove the supernatant and test it.
Alternatively, store it at -20°C or -80°C.
However, repeated freezing and thawing should be avoided.

3.
Tissue Homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove any residual blood (lysed red blood cells in the homogenate will affect the measurement results).
Weigh the tissue and mince it.
Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS.
The specific volume can be adjusted according to experimental needs and recorded.
It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice.
To further lyse tissue cells, the homogenate can be sonicated or repeatedly freeze-thawed.
Finally, centrifuge the homogenate at 5000×g for 5-10 minutes.
Remove the supernatant for analysis.

4.
Cell culture supernatant or other biological specimens: Centrifuge at 1000×g for 20 minutes.
Remove the supernatant for testing, or store at -20°C or -80°C, but avoid repeated freezing and thawing.
Note: Hemolysis of the specimen will affect the final test results, so hemolyzed specimens are not suitable for this test.

Reagent Preparation
After removing the kit from the refrigerator, allow it to equilibrate to room temperature before use.
Dilution of 20× Wash Buffer: Dilute 1:20 with distilled water, i.e., add 1 part 20× Wash Buffer to 19 parts distilled water.

Procedure
1.
Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 20 minutes.
Seal the remaining strips in a ziplock bag and return them to 4°C.

2.
Set up standard wells and sample wells.
Add 50 μL of standard solution of varying concentrations to each standard well.

3.
Add 50 μL of the sample to be tested to the sample wells; leave blank wells untouched.

4.
Add 100 μL of horseradish peroxidase (HRP)-labeled detection antibody to each standard and sample well, except for the blank well.
Seal the wells with sealing film and incubate at 37°C in a waterbath or incubator for 60 minutes.

5.
Discard the liquid, pat dry on absorbent paper, and fill each well with wash buffer (350 μL).
Let stand for 1 minute, then discard the wash buffer and pat dry on absorbent paper.
Repeat this process five times (a plate washer can also be used).

6.
Add 50 μL each of substrates A and B to each well and incubate at 37°C in the dark for 15 minutes.

7.
Add 50 μL of stop solution to each well.
Within 15 minutes, measure the OD value of each well at a wavelength of 450 nm.

Calculation of Experimental Results:
Using the OD value of the measured standard as the horizontal axis and the concentration of the standard as the vertical axis, plot a standard curve on graph paper or using relevant software.
Obtain a linear regression equation.
Substitute the OD value of the sample into the equation to calculate the sample concentration.
This figure is for reference only: Standard Curve.

Sensitivity The minimum detection concentration is less than 0.1 mU/L.
Species Reactivity Rat
Theory The kit uses a double-antibody one-step sandwich enzyme-linked immunosorbent assay (ELISA). Samples, standards, and HRP-labeled detection antibodies are added sequentially to microwells pre-coated with a rat thyroid-stimulating hormone (TSH) capture antibody. The assay is incubated and thoroughly washed. The color is developed using the substrate TMB, which converts to blue under the catalysis of peroxidase and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of rat thyroid-stimulating hormone (TSH) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration.
Detection Type Used for in vitro quantitative detection of rat thyroid-stimulating hormone (TSH) content in serum, plasma, tissue homogenate and related liquid samples.
Composition

Name

96 T

Remarks

Microwell enzyme plate

12 holes×8 strips

None

Standard product

0.3mL*6 tube

None

Sample diluent

6mL

None

Detection antibody-HRP

10mL

None

20×Wash Buffer

25mL

Dilute according to instructions

Substrate A

6mL

None

Substrate B

6mL

None

Stop solution

6mL

None

Sealing film

2 Zhang

None

Remarks:

1. The concentrations of the standard substances are: 20, 10, 5, 2.5, 1.25, and 0.625 mU/L.

2. After testing a large number of normal specimens, the normal concentration values of the specimens are all within the detection range provided by the kit. During the experiment, 50 μL of sample can be directly sampled. If some sample values exceed the maximum standard concentration, the sample can be appropriately diluted with sample diluent before the experiment.

General Notes

1. Strictly follow the specified incubation time and temperature to ensure accurate results. All reagents must reach room temperature (20-25°C) before use. Refrigerate reagents immediately after use.

2. Improper plate washing can lead to inaccurate results. Ensure that the liquid in the wells is as dry as possible before adding substrate. Do not allow the microwells to dry out during incubation.

3. Remove any residual liquid and fingerprints from the bottom of the plate, otherwise it will affect the OD value.

4. The substrate developer solution should be colorless or very light in color. Substrate solution that has turned blue should not be used.

5. Avoid cross-contamination of reagents and specimens to prevent erroneous results.

6. Avoid direct exposure to strong light during storage and incubation.

7. Allow the sealed bag to equilibrate to room temperature before opening to prevent water droplets from condensing on the cold plate strips.

8. No reaction reagents should come into contact with bleaching solvents or the strong fumes emitted by bleaching solvents.

Any bleaching component will destroy the biological activity of the reagents in the kit.

9. Do not use expired products.

10. If there is a possibility of disease transmission, all samples should be managed properly and the samples and detection devices should be handled according to the prescribed procedures.

Storage Temp. Unopened test kit, stored at 2-8°C, has a shelf life of 6 months.
Test Range 0.625 mU/L – 20 mU/L.
Shipping Notes
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Exchange/Return Notes
  • We offer a 30-day return/exchange service after receiving.
  • Final sale items are not eligible for returns or exchanges.
  • To process your return/exchange, please contact us at [email protected]
  • Please click here for more details>>> Return & Exchange Policy
SKU: 27148892891

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Khalsa Family
Belleville, US
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Better than you imagined...
I bought a 4'x6' from Safavieh 10 years ago and just received its replacement. I've slept on it every night and meditated on it every day. In short, I love it. It's the most comfortable surface I can imagine. Here's what you need to know. A sheepskin is just that, animal pelt(s). The 4x6 is four pelts sewn together. It will have a sheepskin smell, which will reduce over time. When it comes, it is rolled up and in a heavy clear poly sleeve, Open the end, not the side! This way if you need to return it, you can roll and slide it right back into the shipping sleeve. The dimensions you buy are approximate. It's a naturally variable product. You might prefer a medical sheepskin if you live in a hot climate. The fur on these is altered to make the hairs shorter. I think I will cover it with a bottom sheet from now on. It might last 20 years that way.
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Reviewed in the United States on October 13, 2024
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Boise, US
★★★★★ 1
PACKAGE STUNK UP BLDG HALLWAY
I went downstairs to pick up the package from our building management office. I could smell it already. The rug was rolled up in clear plastic with one end exposed/open. From what I could touch, the rug felt soft and the color was good. But I was embarrassed to have received this and then bring it into the elevator with me. The smell was horrendous, like sewage or fecal matter. BACKSTORY: I had ordered 2 different sheepskin rugs from 2 different brands. My first time buying sheepskin. The other has a very faint animal/hide smell that is detected only when right up against it. This Safavieh rug, however, was for a different location in the house. Even without the first rug for comparison, the smell of this one is unbearable. I've bought many wool and polypropylene rugs from Safavieh. So I did not expect a negative experience.
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Reviewed in the United States on September 25, 2020
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Patricia Jordan
New York, US
★★★★★ 5
Great product: thick and cozy
Size: Single Pelt/2 ft x 3 ft, Color: Natural (Ivory Whiite)
This is a lovely, thick sheepskin. I’m using it to drape over a chair. It’s very soft. The color is a natural white. Arrived quickly. The price was great for such a wonderful sheepskin.
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Nate B
Charlottesville, US
★★★★★ 5
Love it!
Size: 2' x 6' (Double Pelt), Color: Linen, Size: 2' x 6' (Double Pelt), Color: Linen
Exceeded all expectations. Bigger than I imagined it would be, super soft and thick, beautiful color (Linen), and no funky smell at all. It seems very durable. After running my hands all over it, no shedding occurred. I have yet to sit on it myself, though, as my cat claimed it right away - didn't even wait for me to remove the tag! He's been there for hours and I'm thinking I've lost my snuggle buddy, so I bought two more in the smaller size so he can have his own on the bed with me at night. I am so amazed by the quality, feel, and color. I can't believe I almost let other reviews deter me from this purchase. 100% thrilled with this beautiful pelt. Absolutely recommend! UPDATE: I received the other 2 purchased. Just as soft, just as thick, consistent color (Linen), no shedding, no smell. They have been in use for several days now without any issues. Love them all - and my snuggle buddy is back! 🥰
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Reviewed in the United States on April 22, 2024
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Sowmi
Natrona Heights, US
★★★★★ 5
It’s wonderful!!
Size: 2' x 3' (Single Pelt), Color: Brown, Size: 2' x 3' (Single Pelt), Color: Brown
The product is wonderful! You know it is real, coz the smell doesn’t go away. I use it to meditate and cannot how nicely it created the perfect comforting barrier to help elevate my experience. I however want to find alternatives coz I stopped using animal products after turning a vegetarian. The size (it is bigger in person than in the pic) and color(brown) are perfect for what I do and doesn’t need much/any maintenance! The white however needs a lot of attention and care.I’m sure it would last a decade and the weight is just right too!
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Reviewed in the United States on April 16, 2026

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