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Description
Human CP ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. Cell Lysis Buffer: Gently wash adherent cells with ice-cold PBS, then trypsinize and collect cells by centrifugation at 1000×g for 5 minutes. Suspension cells can be collected directly by centrifugation. Wash collected cells three times with ice-cold PBS and resuspend in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freeze-thaw cycles or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and remove the supernatant for analysis. Cell Culture Supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for analysis or store at -20°C or -80°C, avoiding repeated freeze-thaw cycles. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP enzyme conjugate are sequentially added to microwells pre-coated with a C-Peptide (CP) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the C-Peptide (CP) content in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human C-Peptide ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | C-peptide (CP) is a short, 31-amino acid polypeptide that connects the A and B chains of the insulin molecule. In the setting of diabetes or hypoglycemia, measuring serum levels of C-peptide can be used to differentiate between conditions with similar clinical features. The first documented use of a C-peptide test was in 1972. However, over the past decade, C-peptide has been discovered to be a bioactive peptide in its own right, with effects on microvascular blood flow and tissue health. C-peptide has been shown to bind to the surface of several cell types, such as neurons, endothelial cells, fibroblasts, and renal tubules. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.15-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenate, cell lysate, cell culture supernatant and other biological fluids |
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4.1 ★★★★★
Based on 1533 reviews
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Product Reviews
★★★★★ 4
Great if you're average height.
Color: Black
Comfortable for the price. The seat could be a bit better, but for a budget friendly ergonomic chair, it's well made and has helped a lot with my back pain caused by my previous desk chair.
Assembly wasn't terribly difficult, though I did have one screw with damaged threads. Luckily they ship spare screws in the kit, so that was a non-issue.
Rollers work great on my small rug and hard flooring and don't tend to tangle cords or cables too much.
Size wise, works great for my frame (5 ft 6 in) but for someone more than a few inches taller than myself, you should probably look at another chair.
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Reviewed in the United States on September 4, 2025
★★★★★ 5
Great chair
Color: Black
I am very happy with my purchase. Easy to assemble but be aware your drill bits included with your set won't fit their screws. However, you can get a fair bit done with the drill and then use tool included to adjust. Super comfortable and exactly the right height for my desk. Highly recommend for quality exceeding price point.
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Reviewed in the United States on May 9, 2026
★★★★★ 1
Review from an "actual" tall person who doesn't weigh 400 pounds.
Color: Black
I recommend this chair.
Firstly, I looked at least dozens of chairs and nearly every review in trying to select a chair. I didn't want to spend $200.
It's confusing because everyone's idea of perfect is of course subjective. One thing, What is tall? I'm 6'6" I think that's pretty tall. Others feel that 6'0" is tall. LOL In the listing, it shows this chair is good for a 6'5" person which I think is pretty accurate. I weigh about 210 pounds and the padding is just fine. One thing I would push manufacturers to do would be to give accurate measurements. Seat height low and high. Back support height. Head rest added to back height etc.! Maybe actual human measurements that the chair is geared toward. Nice to know that 400 lbs is cool but that doesn't tell me much.
My primary goal was to find a chair I could sleep in because I recently had a
back injury and sleeping on my bed, while fine, resulted in intense pain trying
to get up in the morning. We'll see what happens but the headrest reaches my head
which was goal one.
The lumbar fits so I don't need to use cushions and pads etc. I found the upper
position of the support to be right for me and it's super comfortable. Plus number
two.
The raised height of the chair fits my long legs (inseam 36") Plus three.
I wanted adjustable arm rests but this one doesn't have that. No biggie, for me the height of the arm rests is pretty much just right. It might be a little high for some.
The back leaning adjustment doesn't lock except for upright. Locking upright is good, and leaning back - for me - doesn't really need to lock.
Being an actual tall person made me really skeptical. I'm relieved that I won't have to take it apart to return it. The assembly was straight forward and pretty easy though I'd suggest not really tightening the arms snugly before getting the back squared away - the alignment is easier if you do that.
I got the chair for $109 with 21% off. It's slightly wobbly yet feels pretty well made. It rolls well enough.
I'm quite satisfied with the purchase and I'll come back if there are updates.
Edit one. I've had it a week now and found I couldn't sleep in it. I think it would be better if the back could recline separately from the seat. There might also be figuring out a way to support my legs due to the back and seat reclining together rather than separately. Two. I kind of wish more now that the arms were adjustable for height and tilt if that makes sense. I have a bar stool next to me on the right in front of my desk that is the right height to relax my right arm while reaching my mouse on the desk so that's my work around there. Three. The casters are kind of basic and don't roll as easily on the floor matt as my former second hand chair - they would probably be fine on a hard surface. I'll probably switch them out. Four: The lumbar support, while mostly in the right place is unpadded and creates kind of a hard spot there. I've re-introduced my lumbar support pillows for comfort there.
All in all, I'm still happy with the $100 purchase. In my experience, being pretty tall always comes with compromise and these notes are not deal breakers for me.
Yea, It can always be more perfect but unless you're in a showroom test driving and willing to spend $300? Right.
UPDATE: I'm really not satisfied with this chair and in fact, I wish I hadn't bought it. Firstly, the pneumatic post that the chair part connects with is wobbly depending on which way it is facing according to which way the legs are aligned. It's like a poor fit and I'm pretty mechanically inclined.
Second and also important. The lock to keep it upright and inclined only worked for a few weeks. For a while, you had to mess with it to engage the upright position. It no longer works.
Worse than that, the lumbar support pad disconnected with the chair and is now floating and after only a month or so, the remaining supports for the Lumbar rest have dug into the fabric on the back of the chair creating holes in the back's fabric. I've tried but there is no way to reconnect that bit without removing the back's fabric as far as I can see.
I'm taking this down to one star for sure. Poor design? Poor manufacturing? Yea. I wouldn't suggest this chair to anyone. If the company comes up with a solution, I'll continue my review.
As for now. Not a recommendation.
Update: Not impressed and kind of crappy. Mentioned is that the so called lumbar thingy disconnected and is unusable. I've got my own pad which seems to help. Also, it's wiggly where the chair connects to the base. Months later, I might toss it and go back to the chair I bought locally and second hand.
Don't buy this chair!
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Reviewed in the United States on March 20, 2025
★★★★★ 5
Great Instructions, Straightforward Assembly
Color: Black
I just received this chair today, so my product review is mostly on the packaging, instructions, and assembly.
The product was well-packaged, and it was obvious when I unboxed it that a lot of care and thought went into packing it in the most efficient way that would also protect the parts. I was positively impressed with the packaging.
I was also incredibly impressed with the instructions and assembly process. I am used to cheaper products where the parts only half fit or important components are missing, or where the instructions were confusing and unclear. This chair's instruction manual was very clear and concise. The diagrams were easy to understand and neat. The screws came labeled and separated and also included the scew types and sizes/lengths, which is handy in case you need to pick up extras at a store somewhere. But they also included one extra of each type of screw, washer, and cover, which I appreciated during assembly. (I didn't find myself worrying what would happen if I dropped a screw, which is something I would normally be distsracted by while working.)
The only things I would take points off for were these:
1. The instructions said the chair came with gloves for assembly, but no gloves were included. That was fine with me, but why mention them if you won't include them? The instructions even later say not to use the chair if not all components were present...but all the important ones were, so I assembled it without gloves (as I probably would have done anyway, since it was for me and not another person).
2. It matters which side of the seat is the front, but the sticker was confusing. The instructions show a sticker placed on the side that is the front, and that is where my sticker was placed. However, the sticker says "FRONT" and has an arrow pointing somewhere. In the instructions this arrow pointed to the front. On my seat, however, the arrow pointed to the back. So am I supposed to believe the sticker placement (it was diagonal, so it looked haphazardly slapped on) or the arrow? On that particular step the piece you are screwing into the seat can fit both ways, but thankfully there is a later step where it becomes obvious which side is the front vs. the back. (The arm rests can only be attached to the back.) So I wish that had been clearer up front, but otherwise it was okay.
Recommendations for assembly:
- To attach the wheels, apply firm, direct, straight-down pressure. I tried screwing them on at first but you just push. Make sure they go in the whole way like the picture shows.
- When they say to tighten the screws to only 60%, it's a helpful tip, because you can't fit the armrests on both the seat and chairback at the same time with the screws completely tightened. Just tighten them enough to fit the pieces on, then later you can tighten up the screws.
So far I find the chair to be very comfortable and I'm exceedingly happy with it. The lumbar support seems like it could potentially be a little chafy or scratchy, but I'll give the chair a chance before deciding if it's problematic or not. Like I said, I only got the chair today, so I can't speak much to its long-term comfort, durability, or longevity. But I was so impressed with how easy and straightforward they made the assembly that I had to write a review to show my appreciation.
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Reviewed in the United States on May 9, 2026
★★★★★ 3
It's okay
Color: Black
Appeared to be a super comfortable chair. Purchased for my mother to work from home and the material is apparently slightly itchy and the arm rest isn't quite comfortable. It was pretty simple to put together but it did take some time.
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Reviewed in the United States on June 11, 2026