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Description
mIHC Dye (TSA480)Product Specification References 1. Dabbs David J. Diagnostic immunohistochemistry (M). Beijing: Peking University Medical Press, 2008. 9. 2. [US] Ed Harlow, David Lane Technical Guide (M). Beijing: Science Press, 2002: 79 80, 105. Stack, E. C., et al., Multiplexed immunohistochemistry, imaging, and uantitation: are view, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis. Methods, 2014; 70 (1): 46 58. 4.
Product Specification
| References | 1. Dabbs David J. Diagnostic immunohistochemistry (M). Beijing: Peking University Medical Press, 2008.9. 2. [US] Ed Harlow, David Lane Technical Guide (M). Beijing: Science Press, 2002: 79-80, 105. Stack, E.C., et al., Multiplexed immunohistochemistry, imaging, and uantitation: are view, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis. Methods, 2014; 70 (1): 46-58. 4. Qian Bangguo Jiao Lei Application of multi-label immunofluorescence staining and Doppler imaging technology in histological research. Chinese Journal of Histochemistry and Cytochemistry, 2017 (4): 373-382. |
| Theory | Multiplex fluorescence immunohistochemical staining is based on the principle of specific binding of antigen and antibody. The secondary antibody labeled with horseradish peroxidase (HRP) is selected to activate the fluorescent dye in the kit and covalently bind the signal to the antigen. In situ multi-target staining of tissues or cells is achieved by multiple rounds of staining cycles with the aid of different fluorescent dye labels. |
| Description | There are complex cells in tissue microenvironment, and the phenotype, state, abundance and distribution of these cells have important biological significance and clinical value. It can be rendered in situ in tissue by means of antibody staining. Immunohistochemical staining is a common technique to study tissue morphology and protein expression in situ. Routine IHC detection can only show a single index, and it is difficult to present the cell composition, state and relationship in the complex tissue microenvironment. This information is crucial to the diagnosis and treatment of diseases! Principle of tyrosine signal amplification technology: Similar to the DAB color development method of conventional immunohistochemistry, TSA technology also uses HRP-labeled secondary antibody. HRP catalyzes the fluorescein substrate added to the system to produce an activated fluorescein substrate, which can be covalently bound to the antigen. Tyrosine is covalently bound to stably covalently bound to fluorescein on the sample. After that, the non-covalently bound antibody is washed away by thermal repair method, replaced with a primary antibody for the second round of incubation, and replaced with another fluorescein substrate, so that multiple labeling can be achieved by reciprocating. |
| Composition | TSA monochromatic fluorescent dye 480, signal amplification reaction solution |
| General Notes | 1. Formalin-fixed wax blocks or glass slides, large tissues or TMA, and sealing wax cannot be obviously damaged. 2. For glass slide samples, the tissue needs to be close to the glass slide to avoid wrinkles, and the glass slide must not be damaged, scratched or stained. 3. The tissue should contain at least more than 1000 cells. 4. The wax block needs to be embedded in solid tumor tissue, and necrotic tumor tissue, fine needle punctures, and cell slice samples will affect the staining effect. 5. The tissue should be fixed with 10% neutral formalin, and the normal fixation time is 18-24h. 6. The thickness of the slice is about 4um, and anti-detachment slides are used. It is recommended that the glass slides be prepared within one week after fixation. 7. Do not add any adhesive in the water bath slide fishing process. The sample should be placed on the front and center of the slide. Place the slide vertically on absorbent paper to remove water, tap gently to remove water droplets, and do not wipe the slide with paper. 8. Place the glass slide on a hot plate at 45 °C for 30min (the natural air-drying time of the glass slide is not less than 1h). |
| Storage Temp. | Fluorescent dyes should be stored in the dark from light at 2-8 ℃, and the validity period is 12 months from receipt of the goods. |
| Applications | It is mainly used for scientific research on immunohistochemical staining of tissues, paraffin sections and TMA chips, and cannot be used for in vitro clinical diagnosis or human experiments. |
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4.9 ★★★★★
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Product Reviews
★★★★★ 4
Really great shirt, really thick material
An unexpected pleasant surprise. Really nice quality, minimal shrinkage, and very few wrinkles out of the dryer. Nice, comfortable fit, and for big-but-not-tall guys, the fitment is very presentable. Sleeves aren't crazy long, and the shirt itself isn't so long that you feel like you're wearing a dress.
These aren't complaints, but I do want to share two notes that were important to me:
1. If you want a breast pocket, be sure to select the right shirt. There are two different versions, and it's not particularly obvious which is which.
2. The material is thick. Really thick. So the shirt is pretty heavy, and it doesn't breathe very well. For me, this is unquestionably a winter shirt. If, like me, you tend to run a bit warm, this shirt can make you feel like you're wearing a parka.
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Reviewed in the United States on October 6, 2023
★★★★★ 5
Great Value from Amazon Essentials
I can’t believe the quality of this shirt given the low price I paid for it. It fits perfectly, looks impressive, and is very comfortable. The shirt has survived two washes so far and looks as good as new. I couldn’t be more satisfied with this purchase.
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Reviewed in the United States on December 11, 2025
★★★★★ 1
Too large, length too long
Size: Medium, Color: Light Grey Vertical Stripe
Runs large, and the length is far too long to wear untucked like in the photos. Always wear a Medium, this fits like a Large. Length of shirt is long and obviously made to be tucked in. You would look ridiculous if you left it untucked. Dont understand why companies still insist on designing shirt to be tucked in when most people dont wear it like that these days
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Reviewed in the United States on April 25, 2026
★★★★★ 5
Great shirt
Size: 6X-Large Big, Color: Pale Pink
Amazing deal
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Reviewed in the United States on March 27, 2026
★★★★★ 5
Traditional Grown Man Shirt
Size: Large, Color: Light Grey Vertical Stripe
Great cotton, classically-style button down shirt for guys not wanting anything slim fit, this shirt size is legitimate. It’s a nice heavyweight cotton shirt. I don’t think I’d say it was wrinkle resistant but it doesn’t take much effort to press this garment.
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Reviewed in the United States on January 20, 2026