SKU: 71513391958

Mouse CA199 (Gastrointestinalcancer Marker-CA199) QuickTest ELISA Kit

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Description

Mouse CA199 (Gastrointestinalcancer Marker-CA199) QuickTest ELISA KitThe Mouse CA199 (Gastrointestinalcancer Marker CA199) QuickTest ELISA Kit measures CA199 in Mouse Serum, Plasma, Cell Culture Supernatant, Cell Lysate or Tissue Lysate. This kit was based on sandwich ELISA method. The experiment lasted 120 minutes. Capture antibody was conjugated to an affinity tag that was recognized by a specific antibody coated on the QuickTest plate. Add the Cap Det Ab working solution into each well, then add the standards and

The Mouse CA199 (Gastrointestinalcancer Marker-CA199) QuickTest ELISA Kit measures CA199 in Mouse Serum, Plasma, Cell Culture Supernatant, Cell Lysate or Tissue Lysate. This kit was based on sandwich ELISA method. The experiment lasted 120 minutes. Capture antibody was conjugated to an affinity tag that was recognized by a specific antibody coated on the QuickTest plate. Add the Cap/Det Ab working solution into each well, then add the standards and pilot samples into individual wells. If the sample contains CA199, a capture antibody-CA199-biotin-detection antibody complex was formed. After incubation, unbound conjugates were removed by wash buffer. HRP-Streptavidin was added. After washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of CA199 in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.

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SKU: 71513391958

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sandy
Houston, US
★★★★★ 1
poor durability
Pattern Name: Squeaky Toy
my dog pulled the strap off in under 10 minutes. The leather is not sturdy and my dog was able to tear it to pieces in 20 minutes
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Reviewed in the United States on December 5, 2025
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Tom walton
Cuba, US
★★★★★ 3
Ok but not Leather
Pattern Name: Tug Rag
I will say it's fairly durable but when the description uses the word leather..one would expect LEATHER, not microfiber. That said..it is thick and pretty durable and the dog does like it ok. Obviously the strap is NOT meant for chewing. This is not a throw it on the ground and let ur dog have it sort of a toy. It's an interactive toy. If left to the dog most would scissor the strap off in the first 10 minutes. Something to keep in mind. In summation..an ok interactive toy but over priced for the material.
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Reviewed in the United States on July 30, 2025
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Holly
New York, US
★★★★★ 5
My dogs favorite toy
The tail lasted about 1 minute, but otherwise it has held up pretty good from my super chewer dog, and he treasures it like a prize. I'll need to get a replacement one as its not indestructible, but overall I'm happy with it.
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Reviewed in the United States on June 6, 2026
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Verified Purchase
Tess66
Charlottesville, US
★★★★★ 5
Dog loves it, sturdy
My dog loves this toy and hasn't destroyed it. Win, win.
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Reviewed in the United States on May 21, 2023
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Michael Clingan
Birmingham, US
★★★★★ 4
Not as tough as the salmon.
We have the salmon from the same manufacturer and it has endured as few other dog toys have. Unfortunately, the squirrel lasted only two days before developing a ripped seam and began loosing stuffing. Maybe a defective toy based on other reviews.
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Reviewed in the United States on January 4, 2025

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